血清羟丁酸测定方法的建立及其在糖尿病酮症酸中毒中的意义.pdfVIP

2001, 26( 2) BULL HUNAN MED UNIV 185 1 2 1 易 斌 , 魏桂芬 , 唐 银 ( 1 中南大学湘雅医院检验科; 2. 检验系95 级湘雅医院实习组, 长沙 4 10008) [ ] 目的: 建立酶速率法测定血清羟 丁酸, 并探讨羟 丁酸在糖尿病酮症酸 毒 的意义方法: 采用酶 速率法在日立7170A 全自动生化分析仪上测定60 例正常人30 例糖尿病人和8 例酮症患者血清 羟 丁酸含量结 1 果: 本方法测定羟 丁酸回收率108% , 线性范围0 ~ 4 . 0mmol . L , 批内CV2 . 2% , 批间CV2 . 6% ; 糖尿病酮症酸 毒患 者血清羟 丁酸明显升高结论: 采用酶速率法测定血清羟 丁酸快速准确线性范围宽, 可作为糖尿病酮症酸 毒 特异和快速的诊断试验 [ ] 羟 丁酸; 糖尿病; 酮病; 酸 毒 [ ] R446112 R5871 [ ] A [ ] 2001) Establishment of method for determining serum betahydroxybutyric acid and its meaning in diabetes ketosis acidism 1 2 1 YI B n , WEI Gu fen , TANG Y n ( 1Dep artment of Clinical Laboratory , XiangyaHosp ital, Centeal South University, Changsha 410008, China; 2. Interne of he Faculty of Medical Examination, Xiangya Medical College, Central South University, Changsha 4 10008, China) Abstract: Objective Th s study was to establ sh enzymerate method for determ n ng serum betahydrox ybutyr c ac d , and to d scuss ts mean ng n d abetes ketos s ac d sm. Methods Enzymerate method was used to determ ne serum betahydroxybutyr c ac d n 60 cases of normals, 30 cases of d abetes , and 8 cases of ketos s ac d sm by H tach 7170A autochem stry analyzer . Results The recovery rate s 108% , the l ner ty extent 04 . 1 0 mmol. L , the coeff c ent of var at on w th nrun s 2. 2% and daytoday s 2. 6% . Conclusion The enzyme rate method for determ n ng serum betahydroxybutyr c ac d save t me, g ves accurate results and has w de l ner ty, so t can used for d