对硝基酚对大型蚤与斑马鱼胚胎的毒性_郑新梅.pdfVIP

2010 年 第5 卷 生 态 毒 理 学 报 Vol . 5, 2010 第5 期，692-697 Asian Journal of Ecotoxicology No . 5, 692-697 对硝基酚对大型蚤和斑马鱼胚胎的毒性 * 郑新梅，丁亮，刘红玲 ，于红霞 南京大学环境学院 污染控制与资源化研究国家重点实验室，南京210093 摘要：采用静态生物毒性试验方法，研究了对硝基酚对大型蚤的急性毒性和斑马鱼胚胎发育影响. 结果表明，大型蚤 幼蚤接触不同浓度的对硝基酚后，活动会受到不同程度的抑制，甚至死亡，48h EC50 值为 2.55mg ·L -1 . 对硝基酚对斑 马鱼胚胎有蓄积致毒作用，最敏感性指标为83h 未孵化、83h 卵凝结；实验还发现对硝基酚具有低浓度促进斑马鱼胚 胎发育和孵化，高浓度抑制发育并延缓孵化的作用. 对硝基酚对大型蚤的毒性与对斑马鱼胚胎的发育影响相比，大型 蚤对对硝基酚反应更为敏感. 关键词：对硝基酚；大型蚤；斑马鱼胚胎；毒性 文章编号：1673 - 5897 （2010）5 - 692 - 06 中图分类号：R99 ，X592 文献标识码：A Toxicity of p -Nitrophenol to Daphnia Magna and Zebrafish （Brachydanio rerio）Embryo * ZHENG Xin-mei ，DING Liang ，LIU Hong-ling ，YU Hong-xia State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093 Received 27 September 2009 accepted 26 October 2009 Abstract ：The static method of biological toxicity test was used to study the effect of p - nitrophenol on Daphnia magna （D. magna）and zebrafish embryos. The results of this study demonstrated that p - nitrophenol remarkably restrained the mobility of D. magna neonates, which may cause subsequent death, and the EC50 value was 2.55mg ·L -1 at 48h. The results also indicated that p - nitrophenol remarkably retarded the development of zebrafish embryos. It can accumulate in the zebrafish embryos. The most sensitive toxicological endpoints in the development of the embryos were 83h non - incubation and 83h coagulation. A hormetic - like biphasic dose response was found in the effects of p - nitrophenol on embryo development. D. magna was also relatively sensitive to the effects of p - nitrophenol exposure