白菜和诸葛菜杂交中的基因组加倍、染色体消除及偏白菜型杂种的产生.pdfVIP

Genome doubling and chromosome elimination with fragment recombination leading to the formation of Brassica rapa-type plants with genomic alterations in crosses with Orychophragmus violaceus MIN LIU, ZAI-YUN LI * National Key Lab of Crop Genetic Improvement, National Center of Crop Molecular Breeding Technology, National Center of Oil Crop Improvement (Wuhan), Huazhong Agricultural University, Wuhan 430070, PRC * lizaiyun@ Abstract In plant distant crosses, non-classical hybrids with unexpected chromosome complements, chromosome elimination and genetic introgression have been documented. Herein hybrid plants were obtained with embryo rescue from intergeneric crosses between Brassica rapa, B. rapa var. chinensis and another crucifer, Orychophragmus violaceus, and mainly displayed a female B. rapa phenotype but also certain O. violaceus or novel characteristics. Variable numbers of chromosomes appeared in somatic cells in the roots of plantlets on medium and ovaries and pollen mother cells (PMCs) and higher cell numbers were recorded in the roots. GISH observations revealed the majority of ovary cells and PMCs to twenty chromosomes of B. rapa with or without individual O. violaceus chromosomes or fragments added or introgressed. AFLP analysis revealed that fragments deleted for B. rapa was much more frequent than novel and O. violaceus fragments and about 50% of them were the same in more than half of F1 plants, and the number of O. violaceus fragments positively correlated with its traits expressed in hybrids. The mechanisms behind the results were the genome doubling and successive elimination of the O. violaceus chromosomes accompanied by the fragment recombination and introgression, producing B. rapa-type plants with modified genetic constitutions and phenotypes.