Protein Purification.ppt

Protein Purification Molecular weight Charge Solubility Affinity Molecular Weight Ultracentrifugation Dialysis Gel filtration SDS PAGE Molecular Weight The lab in week 6 and 7 will involve separating a protein mixture by molecular weight using 2 methods: gel filtration and SDS PAGE Gel filtration separates by the native molecular weight SDS PAGE separates by the subunit molecular weight Gel Filtration This method relies on a column of beads of a specified pore size. This is known as a molecular sieve. Proteins (and other macromolecules) above a certain cut-off size cannot fit into the pores a