第六届竞赛一等奖范文.pdfVIP

藜麦干旱胁迫生理及NO 缓解盐胁迫机理研究 摘要：以品种“Tomico ”为试验材料，研究了干旱胁迫及复水对藜麦生理特性的影响；同时用NO 供体硝普钠（SNP）作为 缓解剂，探讨了NO 对盐胁迫下藜麦幼苗生长及生理生化指标的影响。研究结果表明，随着干旱胁迫时间延长 （0、5、10、 15、20 d ），藜麦生长受到明显抑制，其相对含水量、叶绿素总量显著下降，可溶性糖、脯氨酸及可溶性蛋白含量显著增加， 超氧化物歧化酶（SOD）和过氧化物酶（POD ）的活性均呈先增后减的趋势。复水后，各指标不同程度地得以恢复，但与对 照相比，大部分指标未能恢复到正常水平。盐胁迫下 SNP 处理促进了藜麦幼苗的生长，显著提高了叶片光合色素、可溶性 糖、脯氨酸以及可溶性蛋白的含量，同时增强了抗氧化酶SOD 和POD 的活性，降低了膜脂过氧化产物丙二醛（MDA ）的 含量。本研究为藜麦抗旱机制及NO 在藜麦耐盐育种和盐碱地栽培利用提供了理论依据。 关键词：藜麦；干旱；复水；盐胁迫；一氧化氮 Study on drought stress physiology and mechanism of NO alleviating salinity stress of quinoa Abstract: Variety “Tomico” was used to investigate the effects of drought stress and rewatering on physiological characteristics of quinoa. Meanwhile, the NO donsor nitroprusside (SNP) was used as a reliever to investigate the effect of NO on quinoa seedling growth and biochemical mechanism under salt tolerance. The result showed that with the prolonging of drought stress time (0, 5, 10, 15, 20 d), quinoa growth was obviously inhibited. Accordingly, the relative water content, total chlorophyll content decreased significantly. However, the soluble sugar, proline and soluble protein content were significantly increased. The activities of SOD and POD, as well as the content of MDA, were increased firstly and then decreased. Each index recovered to certain degree after rewatering. However, most of the indicators, compared with the control, failed to recover to the normal level. The suitable concentration of SNP promoted the quinoa seedling growth under salt stress. The contents of photosynthetic pigment, soluble sugar, proline and soluble protein were significantly increased. Also, the activities of SOD and POD increased obviously, while the content of MDA decreased significantly. This study provided a theoretical basis for revealing drought resistance mechanism, illuminating NO on quinoa salt tolerance breeding, and ex