鼻咽癌细胞ClC-3在细胞周期中表达.pdfVIP

230 Acta Physiologica Sinica, April 25, 2004, 56(2): 230-236 Research Paper ClC-3 expression in the cell cycle of nasopharyngeal carcinoma cells 1,* 1 2 WANG Li-Wei , CHEN Li-Xin , Tim JACOB 1 Department of Physiology and Laboratory of Cell Biology, Guangdong Medical College, Zhanjiang, Guangdong 524023, China 2 School of Biosciences, Cardiff University, Cardiff CF10 3US, UK. Abstract : The immunofluorescence approach, the confocal microscopy and the patch-clamp technique were used to investigate the expression of ClC-3 (one of the candidates of volume-activated chloride channels) and its relationships with the volume-activated chloride current and the capacity of regulatory volume decrease (RVD) in the cell cycle of nasopharyngeal carcinoma cells (CNE-2Z cells). The results indicated that CNE-2Z cells expressed ClC-3. ClC-3 was located predominantly inside the cells but not in the membrane. Both the expression level and the distribution of ClC-3 were cell cycle dependent. ClC-3 expression was low in G1 but high in S phase. The cells in G2/M phase possessed an intermediate level of the expression. ClC-3 expression level was negatively correlated to the RVD capacity and amplitude of the volume-activated chloride current in the cell cycle. The results suggest that ClC-3 may be an important factor in the regulation of cell cycle progression, but that it is probably not the chloride channel associated with RVD in these cancer cells. Key words: tumour cells; ClC-3 protien; chloride channels; gene expression; cell cycle ClC-3 1,* 1, Tim JACOB2 1 , 524023; 2