酵母双杂交筛选原癌基因proto_Dbl上游调控分子.pdfVIP

20 10 37 5 # 49 9 # DOI: 10 . 3 971/ j . issn . 100 0- 857 8. 2 010. 05 . 0 03 prot o- Db 唐秋琳, 赵晓斐, 陈向征, 刘 明, 陈 济, 毕 锋 Screening of Upstream Regulators of Proto-Dbl Oncoprotein by Yeast Two-hybrid System T A NG Q iu- in, ZH AO Xiao- f ei, CH EN X iang- zheng , L IU M ing , CH EN J i, BI F eng Div ision of A bdominal Cancer and the Laboratory of Signal transduction Molecular Targeting Therapy , West China H osp ital, Sichuan niversity, Chengdu 610 04 1, China Corresp onging A uthor: BI Feng, E-mail: bif eng@ Abstract: Ob ective T he V L domain truncat e mutatio n cou d dir ect y cause the activ ation of pr oto- Db and the ma ig nant t ransfo rmation . Methods In order to c ar ify the activ ation mechanism o f pro to- Db , yeast tw o- hybrid sy stem w as u sed to screen the pro teins inter acted w ith the V L domain in the human f e- ta brain cDN A ibr ary taking V L domain as the bait . Results 7 po sitive c o nes w hich interact w ith pr o- to- Db- V L do main w ere successfu y harvested . Conclusion T his disco ver y ays the fo undat ion fo r un- cov ering of the reg u ato ry mechanism of prot o- Db . Key words:O nco gene; Db ; Y east tw o- hy br id system : 探讨 VL domain 对Db 癌基因活性的调控机制 本文通过酵母双杂交的方法, 以 pr oto- Db 的V L do main 为诱饵蛋白对人胎脑文库进行筛选 研究共获得7 个能与 V L domain 相互作用的蛋白 Pr oto- Db V L domain 相互作用蛋白的发现将为揭示pro to- Db 的功能奠定基 础 : 原癌基因; Db 癌基因; 酵母双杂交 : R733 . 4 : A : 10 00- 857 8( 20 10) 0 5- 0499- 04 0 ( ) , , , ,