Figure I..ppt

* Figure I. FPLC FXN 29 33 34 35 36 29 33 34 35 36 + D-4F Sc D-4F STOKES DIAMETER nm 17 12.2 10.4 8.2 7.1 HDL PEAK HDL PEAK C C P C C P 30K Figure II. F i r s t d i m e n s i o n Second dimension W a t e r C o n t r o l + D - 4 F 9 . 5 8 . 2 7 . 1 [ n m ] Pre-beta HDL Pre-beta HDL 0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 20 30 40 0.0 10.0 20.0 0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 20 30 40 0.0 10.0 20.0 CHOLESTEROL, mgr / DL CHOLESTEROL, mgr / DL PON ACTIVITY, U/ mL PON ACTIVITY, U/ mL FRACTION # FRACTION # CHOLESTEROL PON ACTIVITY CHOLESTEROL PON ACTIVITY Sc D-4F + D-4F Figure III. MIGRATED MONOCYTES PER FIELD 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14 No Addition Control LDL + hHDL Assay Controls 50 μg HDL Cholesterol 10 μg CCP Cholesterol P=0.01 P=0.01 16 LDL + D - 4F CCP LDL + Chow Control CCP LDL + D - 4F HDL LDL Chow Control HDL LDL + Figure IV. Figure V. 0 5 10 15 20 25 30 35 Control human HDL Sc D-4F D-4F % Cholesterol Efflux from Macrophages 0.1% BSA P0.01 Murine HDL, 50 mg LOOH Per Cholesterol A B C D E LOOH Per Cholesterol D - 4F Chow Pre- Beta HDL * 0.00 0.00 D - 4F 0.00 Chow FPLC Fractions after Pre-beta HDL 0 1.5 3.0 4.5 6.0 0 1.5 3.0 4.5 6.0 Sc - D - 4F D - 4F Chow 0 2 4 6 8 10 12 VLDL/IDL * Sc - D - 4F D -4F Chow LDL * - D 4F Chow HDL Sc D - 4F 0 2.0 1.0 0.5 1.5 2.5 0 2 4 6 8 10 12 14 16 14 * Figure VI. Sc - D - 4F Sc - D - 4F 0 10 20 30 40 50 60 70 PON units Water Control Sc D-4F D-4F * Figure VII. *