大鼠空肠黏膜上皮细胞酪酪肽受体的分布和特性.docVIP

大鼠空肠黏膜上皮细胞酪酪肽受体的分布与特性 　　作者：闫媛，董 蕾 作者单位：1. 西安交通大学医学院第二附属医院消化内科，陕西西安 710004;2. 西安市中心医院消化科，陕西西安 710004 　　【摘要】 目的 检测正常大鼠空肠黏膜酪酪肽(PYY)受体的分布并测定反映酪酪肽受体特性的基本参数，初步阐明酪酪肽的作用位点和作用特点。方法 用125I标记酪酪肽并对其进行受体放射分析，测得酪酪肽受体的平衡解离常数，最大结合容量及Hill系数。结果 正常大鼠空肠黏膜上皮细胞酪酪肽受体的平衡解离常数(Kd)为(386.69±129.95)pmol/L，最大结合容量(Bmax)为(303.21±116.85)fmol/mg蛋白;Hill系数为1。酪酪肽受体为高亲和力受体。结论 正常大鼠空肠黏膜上皮细胞有酪酪肽受体的分布，酪酪肽受体之间无正负协同效应;酪酪肽受体对PYY具有高亲和性;可能有PYY直接与空肠黏膜上皮酪酪肽受体结合而直接抑制空肠黏膜的分泌。 　　【关键词】 酪酪肽受体;酪酪肽;平衡解离常数;最大结合容量;胃肠激素 　　in rat jejunum epithelial cellYAN Yuan1,2, DONG Lei1 　　(1. Department of Gastroenterology, the Second Affiliated Hospital, 　　Medical School of Xian Jiaotong University, Xian 710004; 　　2. Department of Gastroenterology, Xian Central Hospital, Xian 710004, China)ABSTRACT: Objective To detect normal rat jejunum epithelial cells to learn about the distribution of peptide YY (PYY) receptor and measured two important parameters of PYY receptor so as to explore the target that PYY binds to and display its physiological function. Methods Receptor radioligand assay was conducted by using 125IPYY to detect normal rat jejunum epithelial cells. We also measured receptor dissociation constant (Kd), the affinity or the ability that the receptor could bind ligand; maximum binding capacity (Bmax), the concentration of binding site; and Hill coefficient. Results There were PYY receptors in normal rat jejunum epithelial cells. Dissociation constant (Kd) of PYY receptor was (386.69±129.95)pmol/L. Maximum binding capacity (Bmax) was (303.21±116.85) fmol/mg protein. Hill coefficient was 1. PYY receptor had high affinity. Conclusion There are distributions of PYY receptors in normal rat jejunum epithelial cells. Kd means that PYY receptor has high affinity. Bmax means that the number of receptors is limited and decides the characters of saturability. We think that PYY directly inhibits secretion by binding to PYY receptor in jejunum epithelial cells when PYY inhibiting jejunum secretion. 　　KEY W