Plackett_Burman实验设计优化餐厨垃圾发酵产燃料酒精的研究.pdfVIP

29 5 Vol. 29,No.5 2008 5 ENVIRONMENTAL SCIENCE May,2008 PlackettBurman 1 2 1, 2* 2 3 , , , , ( 1, 150090; 2, 100083; 3 , 100029) :, , PlackettBurman . , , ,. 100 Ug. 100Ug 100Ug ,53 gL, 10%, 44%. , 15 , . ,,. :; ;; PlackettBurman : 705 :A 2008) Optimization of Fuel Ethanol Production from Kitchen Waste by PlackettBurman Design 1 2 1, 2 2 3 MA Hongzhi , GONG Lijuan , WANG Qunhui ,ZHANG Wenyu , U Wenlong ( 1School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China; 2School of Civil and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China; 3China Academy ofUrban Construction, Beijing 100029, China) Abstract:Kitchen garbage was chosen to produce ethanol through simultaneous saccharification and fermentation ( SSF) by Zymomonas mobilis . PlackettBurman design was employed to screen affecting parameters during SSF process. The parameterswere divided into two parts, enzymes and nutritions. None of the nutritions added showed significant effect during the experiment, which demonstrated that the kitchen garbage couldmeet the requirement of the microorganism without extra supplementation. Protease and glucoamylase were determined to be affecting factors for ethanol production. Single factor experiment showed that the optimum usage of these two enzymes were both 100 Ug and the corresponding maximum ethanol was determined to be 53 gL. The ethanol yield could be as high as 44%. The utilization of kitchen garbage to produce ethanol could reduce threaten of waste as well as improve the protein content of the spent. This method could save the ethanol production cost and benefit for the recycle of kitchen garbage. Key words: kitchen garbage; simul