大肠杆菌tRNA_Leu_的提取_纯化和性质研究.pdfVIP

14 6 Vol. 14,No. 6 199812 Chinese Journal of Biochemistry and Molecular Biology ec. 1998 Leu * tRNA 涂华民　孟建新　杨燕生* * ( , 510275) 李　勇　刘　文　王恩多 ( , 200031) Leu Leu 采用高表达大肠杆菌tRNA 菌株提取、纯化了亮氨酸等受体转移核糖核酸tRNA1 和 Leu Leu Leu 2 1 1 tRNA . 利用稳态动 学手段研究了tRNA 及脱镁tRNA 在不同稀土离子作用下与纯化 Leu 亮氨酰-tRNA 合成酶的氨酰化作用. tRNA1 与亮氨酰-tRNA 合成酶的结合及催化效率均受 参与稀土离子的影响, 表观K m 值有较明显的变化. 结果表明, 亮氨酰-tRNA 合成酶催化的 Leu 2+ tRNA1 氨酰化反应所需Mg 能够被稀土离子取代,但亲合性能不同. :亮氨酸转移核糖核酸, 氨酰化反应, 亮氨酰-tRNA 合成酶, 稀土离子 Preparation Purification and Properties of, Escherichia coli tRNALeu Tu Hua-MinMeng Jian-XinYang Yan-Sheng ( , , 510275) D ep artme nt of Chemistry Z hong shan Univ ersity Guang z hou Li YongLiu WenWang En- uo (S tate-key L abor atory of M ole cular B iology ,Sh ang hai nstit ute of B ioche mistry ,A cad emia S inica,S hang hai 200031) - Abstract The biological activity of a nucleic acid is instinctively associated with its three di- mensional structure Metal ions affect the secondary and tertiary structures stabilities and. , ,