大豆甙元对去卵巢大鼠雌二醇.docVIP

大豆甙元对去卵巢大鼠雌二醇、维生素D和骨矿沉积的影响 首席医学网 ???? 2009年07月06日 17:38:22 Monday?? 82 作者：高美 金邦荃 张祎 汤丹 周耀民 詹巍????作者单位：南京师范大学食品科学与营养系，江苏 南京 210097  【摘要】? 目的 研究不同剂量大豆甙元对去卵巢大鼠雌二醇(E2)激素水平和骨矿沉积的影响。方法 60只3月龄雌性大鼠分为6组：对照组、模型组、4个去卵巢+大豆甙元组，每日分别灌服10 mg/kg蒸馏水和25、50、75、100 mg/kg大豆甙元；9月龄时处死，分别测定骨灰分、Ca、P、Mg、Zn和血清中E225羟基维生素D(25OHD)及骨钙素(BGP)。结果 服用大豆甙元后，去卵巢大鼠血清E2水平未升高，但25OHD和BGP水平恢复正常，从而增加了骨Mg和Zn沉积。结论 大豆甙元通过调高(25OHD)及BGP水平，帮助骨矿沉积，可获得与雌激素替代疗法(ERT)近似的临床效果，但不诱导雌激素高表达，使用安全性高。 【关键词】? 雌激素；维生素D；骨矿沉积；大豆甙元；去卵巢大鼠 第一作者：高美(1983)，女，在读硕士，主要从事营养学研究。Effects of daidzein on the expressions of estradiol and 25OHD and? bone minerals deposition in ovariectomized rats 　　GAO Mei, JIN BangQuan, ZHANG Yi, et al. 　　Department of Food Science and Nutrition, Nanjing Normal University, Nanjing 210097, Jiangsu, China ??? 【Abstract】Objective? To evaluate the daidzein on the expressions of estradiol (E2) and 25OHD and bone minerals deposition in ovariectomized (OVX) rats.Methods? 60 3monthold female rats were divided into normal control, OVX and four different doses of daidzein (25, 50, 75, 100 mg/kg BW) treatment groups. At the end of trial (age of 9month), all the rats were killed to collect their leg bone and blood as samples to test bone mineral, Ca, P, Mg, Zn in bone by atomic absorption method or Ca, P, E, 2,5OHD and BGP in serum by ELISA. Results? The serum E2 level was not changed in OVX rats with or without daidzein. The Mg, Zn deposition in bone? were increased because the serum levels of 25OHD and BGP were recovered in daidzein treatment groups. Conclusions? Daidzein could improve the bone minerals deposition through upregulating the levels of 25OHD and BGP, which is similar to ERT but more safety because the level of E is not increased.??? 　　【Key words】Estradiol; 25OHD; Bone minerals deposition; Daidzein; Ovariectomized rats 　　随着年龄增长及更年期的到来，女性卵巢功能逐渐衰退，卵泡退化导致雌激素(E2)水平陡然下降，内源性E2的匮乏，使中老年女性骨代谢平衡遭到破坏，骨吸收大于骨形成，特别是承重骨出现进行性骨量丢失、骨质疏松，甚至骨折。雌激素替代疗法(ERT)治疗更年期骨质疏松已经得到充分的肯定，但其也具有潜在的副作用〔1～3〕。作为植物雌激素的大豆