人BDNF与GFP基因共表达慢病毒载体的构建及神经干细胞转染.pdfVIP

( ) # 1250# Jou rna l of Sh anghai J iao tong Un ivers ity( M ed ical Sc ien ce) V ol. 28 N o. 10 O ct. 2008 0258- 5898( 2008) 10- 1250 - 04 # 论 著# BDNF GFP 1 1 1 2 3 1 , , , , , ( 1. , 20 1900; 2. , 200031; 3. , 200032) 目的 ( hBDNF ) ( GFP ) ( N SCs) 方法 , hBDN F GFP pW PXL-M OD ( pW PXL-G FP- IRE S-GFP ) , pW PXL-hBDNF- IRE S-GFP, M luÑEcoRÑ pW PX L-hBDNF- IRE S-GFP HEL PER VSVG 293T , pW PX L-hBDNF- IRE S-GFP N SCs, 结果 pW PX L-hBDNF-IRE S-EGFP M luÑ EcoRÑ; G enb ank hBDN F 293T , 0. 1 @ 109 ~ 1 @ 109 TU /m LpW PXL-hBDNF-IRES-GFP N SCs , , N SCs; 结论 hBDNF GFP N SCs, ; ; ; Q 7 8 A Construction of bicistron ic lentiv irus vectors carry ing hBDNF and GFP genes and transfection of neural stem cells 1 1 1 2 3 1 L IU Y ong , CHEN E r-tao , FENG D ong-fu , LIU T ian-j in , WANG Y ang , PAN D ong-chao (1. Department of N eurosurgery, The Third Peop lepsH ospita,l School of M edicine, ShanghaiJiaotong niversity, Shanghai 201900, China; 2. Institute of B iochemistry and CellB iology, Shanghai Institutesf orB iologicalSciences, ChineseAcademy of Sciences, Shanghai 200031, China; 3. Department of Anatomy and H istology and Embryology, ShanghaiM edical College of Fudan niversity, Shanghai 200032, China) Abstract Obj ective T o construct hum an brain-derived neurotroph ic factor ( hBDNF ) and green fluorescen t protein ( GFP) co-express