茶园叶面真菌和茶云纹叶枯病原菌的互作关系研究.pdfVIP

茶叶科技创新与产业发展学术研讨会论文集 茶园叶面真菌与茶云纹叶枯 病原菌的互作关系研究 王迎春，李春华，王云，唐晓波 （四川成都 四川省农业科学院茶叶研究所） 摘要： 〔目的〕探索茶园叶面分离的真菌与茶云纹叶枯病原菌的相互作用关系。〔方 法〕平板对峙培养法，将叶面真菌与茶云纹叶枯病原菌进行对峙培养，对比研究从生 态茶园和非生态茶园叶面分离的真菌与茶云纹叶枯病原菌的相互作用。〔结果〕根据 叶面真菌对病原菌的作用，将病原菌分为拮抗菌类、共生菌类和协生菌 3 类。两类茶 园中这三类菌群的构成比例无明显差异，非生态茶园为 72.9% 、5.1%和 22.0% ，生态茶 园为 72.5% 、4.4% 、和 22.1% 。非生态茶园中弱拮抗作用的菌株为优势菌群，比生态茶 园高 9.8% ，而有强拮抗作用的菌株的比例非生态茶园比生态茶园中低 7.4% 。两类茶园 中对病原菌有明显促进作用的菌株构成也不相同，生产茶园中为 6.8% ，而非生态茶园 中为 5.9% 。〔结论〕该研究为茶园病害的生物防治提供了理论依据。 关键词：叶面真菌；茶云纹叶枯病原菌；茶园；对峙培养；互作 Study on the Interaction Between Fungi on Leaves from Ecological Tea Garden and Ordinary Tea Garden and Colletotrichum camellia WANG Ying-chun ，LI Chun-hua ，WANG Yun ，Luo Fan, TANG Xiao-bo (Tea Research Institute, Sichun Academy of Agriculture Sciences, Chengdu, China 610066) Abstract [Objective] The aim was to explore the interaction between fungi separated from leaves of tea gardens and Colletotrichum camellia . [Method] The method of plate confrontation culture was used to culture the fungi and C. camellia. The interaction between fungi separated from leaves of ecological tea garden and ordinary tea garden and C. camellia were studied through comparison. [Result] According to the effect of leaf fungi on the pathogens, the pathogen could be divide into 3 kinds of antagonistic fungi, symbiotic fungi and assistant fungi. The composing proportion of 3 kinds of fungi in the 2 tea garden had no obvious difference. The 3 kinds of fungi in the ordinary tea garden were72.9%, 5.1% and 22.0%, resp. and that in the ecological tea gardens were72.5%, 4.4% and 22.1%, resp. The strains with weak antagonism in the ordinary tea garden were dominant strains, which were 287 茶叶科技创新与产业发展学术研讨会论文