新抑癌基因pig11高表达hepg2细胞株建立.pdfVIP

35 5 2007 9 ( ) Vol. 35 No. 5 Journal of Nanhua University Medical Edition Sep. 2007 PIG11 Hep G2  , , , , (, 421001)  : PIG11 ,PIG11 Hep G2 , PIG11 pcDNA3. 1ΠNT - GFP - PIG11 PCR PIG11 ,PIG11 cDNA pLXSN - PIG11 PCR DNA lipofectamineTM 2000 PA317 , pLXSN - PIG11 Hep G2 ,PIG11 Hep G2 384 bp PIG11 ,PIG11cDNA Genbank ,PA317 Hep G2 ,RT - PCR Hep G2 PIG11 mRNA PIG11 Hep G2 , : PIG11 ; ; Hep G2 ( ) :R730. 1 ;R735. 7  :A !:1672 - 7444 2007 05 - 0653 - 03 Recombinant PIG11 Gene Retroviral Vector Construction and Its High Expression in HepG 2 Cells WU Yan , WANG Xiao - juan , LIU Xiao - min , et al ( Cancer Research Institute , Nanhua University , Hengyang , Hunan 421001 , China) Abstract : Objective To construct the human PIG11 gene retroviral vector and obtain a Hep G2 cell line high expressing PIG11gene by infection , which can be used for the further research on the function of PIG11 gene in liver cancer cell. Methods PIG11 gene was amplified by PCR from expression vector pcDNA3. 1ΠNT - GFP - PIG11 , this gene fragment insert was put into retroviral vector pLXSN , then this new vector pLXSN - PIG11 was identified by PCR and DNA sequencing analysis. This vector was transfected into packing