黄芪对阻塞性黄疸大鼠白细胞介素-2和可溶性白细胞介素-2受体表达的影响.docVIP

黄芪对阻塞性黄疸大鼠白细胞介素-2及可溶性白细胞介素-2受体表达的影响 　　作者：朱振红，杨聪颖，孔庆云，巨卫平 作者单位：徐州医学院附属连云港市第一人民医院消化内科，江苏 连云港 　　【摘要】 目的 通过建立阻塞性黄疸大鼠动物模型，应用黄芪制剂进行干预，探讨黄芪对实验性阻塞性黄疸大鼠免疫功能受损的保护作用。方法 造模3组：假手术组、阻塞性黄疸组、黄芪干预组。手术后10天检测各组肝功能及血白细胞介素-2(IL-2)、可溶性白细胞介素-2受体(sIL-2R)表达水平。结果 与假手术组和黄芪干预组比较，阻塞性黄疸组大鼠IL-2水平降低，sIL-2R水平升高，机体免疫功能受损明显，差异有统计学意义(P0.05或P0.01)。结论 黄芪可提高阻塞性黄疸大鼠IL-2水平，降低sIL-2R水平，增强机体免疫功能。 　　【关键词】 阻塞性黄疸;黄芪;白细胞介素2;可溶性白细胞介素2受体;动物模型;大鼠 　　The influence of astragalus on the expression of interleukin-2 and soluble 　　interleukin-2 receptor in experimental obstructive jaundice in rats 　　ZHU Zhenhong, YANG Congying,KONG Qingyun,JU Weiping 　　(Department of Gastroenterology, The Affiliated Lianyungang First People′s Hospital of Xuzhou Medical College, 　　Lianyungang, Jiangsu 222002, China) 　　Abstract： Objective To investigate the protective effect of astragalus on the immune system injury in obstructive jaundice by establishing obstructive jaundice rat model and by administering astragalus agents for intervention, thus offering a potential therapeutic strategy to obstructive jaundice diseases. Methods 30 male Wistar rats were divided into three groups at random and on the average. A: sham operation group; B: obstructive jaundice group; C: OJ+Astragalus group. Ten days later the rats were sacrificed and the serum was obtained to detect the ALT, bilirubin, IL-2 and sIL-2R in the three groups. Results Compared with groups A and C, IL-2 level in group B decreased and sIL-2R level in group B increased, with marked evidence of injury to the immune functions. There was significant statistic difference between group B and C (P0.05 and P0.01, respectively). The hepatic functional parameters in group C were lower than those in group B (P0.05). Conclusion Astragalus can enhance bodys immune function by increasing the level of IL-2 and decrease the level of sIL-2 R. 　　Key words: obstructive jaundice; astragalus; interleukin-2; soluble interleukin-2 receptor; animal model; rat 　　阻塞性黄疽是一