胰腺癌Syk表达和病理分期及血管生成的相关性.docVIP

胰腺癌Syk表达与病理分期及血管生成的相关性 　　作者：张红雨,费立明,王长亮,赵文华,张波　　作者单位：山东大学齐鲁医院干部保健科， 济南 　　【摘要】 目的 研究胰腺癌中Syk的表达及其与病理分期、血管生成的关系。方法 收集胰腺癌组织40例，另选9例慢性胰腺炎、8例正常胰腺组织标本为对照。应用免疫组织化学方法和RTPCR检测Syk蛋白的表达，形态计量学图象分析法测定微血管密度(MVD)。结果 慢性胰腺炎标本中Syk阳性率为77.8%(7/9), 8例正常胰腺组织中Syk全部阳性表达;Syk蛋白在胰腺癌组织中呈低表达，阳性率为27.5%(11/40);Syk阳性率与肿瘤的发生部位无关，而与TNM分期、分化程度和淋巴结转移有关(P均0.05)。PCR结果显示Syk在胰腺癌中呈低表达(P0.01)。MVD与胰腺癌的组织分化程度、TNM分期相关(P0.01)，胰腺癌组织中Syk蛋白表达阴性者MVD高于阳性者(P=0.000)。结论 Syk在胰腺癌中表达减低或缺失，与胰腺癌的发展相关，可能参与胰腺癌的血管生成和淋巴结转移的过程。 　　【关键词】 脾酪氨酸激酶;血管生成;病理学;胰腺肿瘤 　　3. Shandong Tumor Hospital, Jinan 250117, China) 　　To study the expression of Syk and its correlations with clinical pathology and angiogenesis in pancreatic adenocarcinoma (PAC). Methods Forty PAC, 9 chronic pancreatitis and 8 normal pancreatic tissues were collected. The expressions of Syk protein in these tissues were assayed by immunohistochemical staining, and also expressions of Syk mRNA in PAC were investigated by realtime PCR. MVD marked by CD34 was detected by morphometric analysis, and the relationship between MVD and clinical pathology of PAC was analyzed. Results The positivity rate of Syk protein in PAC, chronic pancreatitis and normal pancreatic tissues was 27.5%, 77.8% and 100%, respectively. There were 29 samples with negative Syk protein, and among them the Syk levels had no relations to the cancer position, but had relations to the TNM classification, tumor differentiation and lymphotic metastasis(P0.05). The MVD was higher in positive Syk proteins than in negative ones (P=0.000). Conclusions The expression of Syk in pancreatic cancer is significantly lower than that in chronic pancreatitis and normal pancreatic tissues and is related to the development of pancreatic cancer. MVD has significant relationship with differentiation and histology types of PAC. 　　Key words: Spleen tyrosine kinase; Angiogenesis; Pathology; Pancreatic neoplasms 　　蛋白酪氨酸激酶(protein tyrosine kinases, PTKs)是一组能催化底物蛋白酪氨酸残基磷酸化的酶蛋白，参与许多信号转导途径，并在控制细胞分化、增殖和扩散中起重要作用。