氧化磷酸化修饰神经丝蛋白M在脊髓缺血再灌注损伤中发挥了保护作用.docVIP

氧化磷酸化修饰神经丝蛋白M在脊髓缺血再灌注损伤中发挥了保护作用 涉及脊髓缺血再灌注损伤组织中的蛋白质种类数以千计，其中可能存在多种影响损伤脊髓神经元存活、轴突再生以及重建功能性突触联系的活性成分。早期脊髓缺血再灌注损伤差异蛋白质呈不同变化趋势，那么差异表达蛋白是否在脊髓缺血再灌注损伤过程中发挥作用？其可能作用机制是什么？来自中国吉林大学杨小玉应用双向凝胶电泳与质谱鉴定方法，分析比较脊髓缺血min再灌注012，24，48h与假手术兔脊髓中的蛋白表达差异神经丝蛋白M蛋白缺血再灌注损伤是过其蛋白磷酸化修饰来实现。 免疫组化染色显示再灌注 24h时，兔脊髓组织中神经丝蛋白M阳性颗粒位于神经元细胞膜和轴突处 Article: Oxidative phosphorylated neurofilament protein M protects spinal cord against ischemia/reperfusion injury, by Haitao Wang1, Su Pan2, Xiaoyu Yang2, Benqing Zhu3, Dalin Wang1 (1 Department of Orthopedic Surgery, Affiliated Hospital of Beihua University, Jilin, Jilin Province, China; 2 Department of Orthopedics, the Second Hospital of Jilin University, Changchun, Jilin Province, China; 3 Department of Orthopedics, People’s Hospital of Tianjin, Tianjin, China) Wang HT, Pan S, Yang XY, Zhu BQ, Wang DL. Oxidative phosphorylated neurofilament protein M protects spinal cord against ischemia/reperfusion injury. Neural Regen Res. 2014;9(18):1678-1687. 欲获更多资讯：Neural Regen Res Oxidative phosphorylated NF-M protects spinal cord against ischemia/reperfusion injury Many proteins are involved in spinal cord ischemia/reperfusion (I/R) injury, affecting neuronal survival, axonal regeneration and reconstruction of functional synaptic connections in the injured spinal cord. Previous studies have shown that neuroflament protein M expression (NF-M) is upregulated in the early stage of spinal cord I/R injury, indicating that this protein may play a role in the injury process. Dr. Xiaoyu Yang and colleagues from the Second Hospital of Jilin University, China compared protein expression in spinal cord tissue of rabbits after 25 minutes of ischemia followed by 0, 12, 24, or 48 hours of reperfusion with that of sham operated rabbits, using proteomic two-dimensional gel electrophoresis and mass spectrometry. In addition, the nerve repair-related NF-M with the unregulated expression was detected with immunohistochemistry and western blot analysis. Researchers found that NF-M expression