致敏小鼠骨髓间充质干细胞体外培养及多向分化功能的测定.pdfVIP

中国病理生理杂志 ChineseJournalofPathophysiology 2010，26(鱼)；03二至 · 1203 · [文章编号] 1000—4718(2010)06—1203—05 致敏小鼠骨髓间充质干细胞体外培养及 多向分化功能的测定 洪冬玲 ， 吴燕峰 ， 许 吕宏 ， 方建培 (中山大学附属第二医院 儿科，生物治疗中心 ，广东 广州510120) [摘 要] 目的：评价异基因脾细胞输注致敏的小鼠骨髓源性间充质干细胞 (MSCs)的体外培养生长能力及 其多向分化功能。方法：应用贴壁培养法体外培养间充质干细胞 ，流式检测其表面标志以及检测其成骨、成脂和成 肌多向分化状况；结果：致敏小鼠骨髓源性MSCs与非致敏小鼠骨髓源性 MSCs比较，形态学无差异且均表达 CD29 、CD105 、CD44和Sca一1；CD34一、CD1lb一；同时在相应的诱导条件下具有向成骨、成脂 、成肌多向分化 的能力。结论：异基因脾细胞输注致敏的小鼠，其骨髓源性 MSCs的形态学和功能与正常小 鼠的MSCs比较评估未 见异常。 [关键词] 致敏小鼠；间充质干细胞；细胞分化 [中图分类号] R363 [文献标识码] A doi：10．3969／j．issn．1000—4718．2010．06．031 Biologicalcharacteristicsand induced differentiation ofbonemarrow —— derivedmesenchymalstem cellsfrom sensitizedmiceinvitro HONGDong—ling ，WUYan—feng ，XUbU—hong，FANGJian—pei (DepartmentofPediatrics，BiotherapyCenter，TheSecondAffiliatedHospitalof SunYet—senUniversity，Guangzhou 510120，China．E—mail；jpfang2005@163．corn) [ABSTRACT] AIM ：Toevaluatethebiologicalcharacteristicsanddifferentiatingpotentialsofbonemarrow—de— rivedmesenchymalstemcells(MSCs)from sensitizedmicebyallogeneicsplenocytetransfusioninvitro．METHODS： Adherentculturemethodwasappliedforculturingthebonemarrow—derivedMSCsfrom sensitizedmice．Thecellmorphol— ogywasexaminedandthesurfacemarkerprofileswereanalyzedbyflow cytometry．Thedifferentiatingpotentialsofthe MSCsintoosteogenic，adipogenicandmyogeniclineageswereexplored．Thebonemarrow—derivedMSCsfrom thenormal micewerecollectedandservedascontrols．RESULTS：Boththebonemarrow—derivedMSCsrfom sensitizedandnormal micewereexhibitedahomogeneousdistinctivemorphologyandwerepositiveofrthesurfacemarkersCD29，CD105，CIM4 andSca一1．negativeinCD34andCD11b．TheabilitiesIlfbothMSCstOdifferentiateintoosteogenic，adipogenicandmy— ogenicpathwaysinthesameconditionwerealsoobserve