山羊MyoD基因家族多态性及和体尺性状的相关性.pdfVIP

HEREDITAS (Beijing) 2007 9 29(9): 1077 ―1082 ISSN 0253-9772 研究报告 DOI: 10.1360/yc-007-1077 MyoD 1, 3 1, 2 1 2 1 1 1 张海军 , 陈宏 , 房兴堂 , 张润锋 , 鲍斌 , 高雪原 , 邵汝英 1. , 221116; 2. , , 712100; 3. , 226100 : 用PCR-SSCP 技术研究了波尔山羊和徐淮山羊2 个群体共 147 个个体MyoD 基因家族中3 个基因座位的 单核苷酸多态性(single nucleotide polymorphism, SNP)。结果表明, 在徐淮白山羊群体中, myf-5 基因座发现有 3 种基因型AA 、AB 和BB , 波尔山羊均为AA 型。在myf-6 基因座和myoD 5′侧翼区基因座, 两个山羊群体均检 测到了AA 和AB 型个体。对山羊myf-5 、myf-6 基因座, myoD 5′侧翼区基因座不同基因型与两品种山羊体尺性 状相关分析表明, myf-5 基因座对管围和管围指数的效应差异显著(P0.05) 。myf-6 基因座对徐淮白山羊体尺性状 的效应均不显著(P0.05), 而对波尔山羊的体高和管围指数效应差异显著(P0.05) 。两个山羊品种myoD 5′侧翼 区不同基因型个体的体尺性状差异均不显著。 : 山羊; myf-5; myf-6; MyoD ; 遗传多态性 Association of variations in MyoD family of genes with body meas- urement traits in goat 1,3 1,2 1 2 1 ZHANG Hai-Jun , CHEN Hong , FANG Xing-Tang , ZHANG Run-Feng , BAO Bin , 1 1 GAO Xue-Yuan , SHAO Ru-Ying 1. Institute of Cellular and Molecular Biology, Xuzhou Normal University , Xuzhou 221116, China; 2. College of Animal Science and Technology, Northwest AF University , Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China; 3. Haimen Middle School of Jiangsu Province, Haimen, Jiangsu 226100, China Abstract: Single nucleotide polymorphisms (SNPs) of hircine myf-5, myf-6 genes and 5 flanking region of the myoD gene (myoD 5 ) were analyzed by PCR-SSCP in Boer goat and Xuhuai white goat. Three genotypes were found at the myf-5 lo- cus of Xuhuai whi