植物苯丙氨酸解氨酶的生物学研究进展.pdfVIP

《现代食品科技》 Modern Food Science and Technology Vol.23 No.7(总 97) 植物苯丙氨酸解氨酶的生物学研究进展 马俊彦 1 1 2 ，杨汝德 ，敖利刚 （1.华南理工大学生物科学与工程学院，广东 广州 510640） （2.江西省南昌市昌北国际机场边防检查站，江西 南昌 330114） 摘要：苯丙氨酸解氨酶（phenylanlanine ammonialyase ，PAL, E.C.4.3.1.5 ）作为植物次生代谢特别是苯丙烷途径的关键酶，与植物 抵抗病原菌入侵有密切关系，具有重要的植物生理意义。本文综述了植物 PAL 的存在与分布、分子结构、酶学性质和作用机制，应 用酶学和分子生物学知识阐述了其表达调控机理，并在基因水平上对PAL 的分子生物学研究进行了展望，旨在为今后PAL 在植物抗 病应用上提供基础数据。 关键词：苯丙氨酸解氨酶；作用机制；表达调控 中图分类号：Q555+.6；文献标识码：A ；文章篇号:1673-9078(2007)07-0071-05 Progress in Biological Research of Phenylanlanine Ammonialyase (E.C.4.3.1.5) 1 1 2 MA Jun-yan , YANG Ru-de , AO Li-gang （1.School of Biosicience and Bioengineering, South China University of Technology, Guangzhou 510640, China ） （2.Nanchang Changbei International Airport Frontier Inspection Station, Nanchang 330114, China ） Abstract: As a critical enzyme in secondary metabolism of plants, especially in the first reaction of the biosynthesis of L- phenylalanine, Phenylanlanine ammonialyase (PAL,E.C.5.3.1.5) has significant biological meaning and strong ability against bacteria. The distribution, molecular structure, enzymatic characters and mechanism of PAL in plants were reviewed. Meanwhile, the regulation mechanisms of PAL was introduced using enzymology and molecular biology. The development of the researches on molecular biology of PAL at the gene level was prospected to provide basic data for the application of PAL in fruit tress disease resistant. Key words: henylanlanine ammonialyase; function mechanism; expression and regulation 植物代谢分为初、次两级代谢，苯丙烷类代谢是 的防御系统，提高植物抗病性化学物质的产