冷藏鲜肉中腐败乳酸菌的分离及鉴定.pdfVIP

年 月 2009 7 检测分析 食食品研究与开发食品研究与开发食品研究与开发品研究与开发 第 卷第 期 30 7 117 冷藏鲜肉中腐败乳酸菌的分离及鉴定 董彩文，白燕红，曹高山，毛多斌，杨公明* 郑州轻工业学院 食品与生物工程学院，河南 郑州 （ 450002） 摘 要：利用稀释平板法从冷藏3 d 的肉样品中共分离出60 株疑似乳酸菌菌株，经过2 次划线分离，然后进行革兰 氏染色、镜检、过氧化氢酶试验、明胶液化试验、硝酸盐还原试验和H S 检验，得到36 株乳酸菌。将分离出的这36 株 2 乳酸菌，经过形态学和生理生化鉴定，确定他们属于5 大类，即清酒乳杆菌，13 株；马里乳杆菌，8 株；消化乳杆菌， 7株；口乳杆菌，4 株；未知乳杆菌，4 株。 关键词：乳酸菌；分离；鉴定 ISOLATION AND IDENTIFICATION OF CORRUPTED LACTIC ACID BACTERIA FROM FRESH MEAT DURING STORAGE IN LOW TEMPERATURE DONG Cai-wen, BAI Yan-hong, CAO Gao-shan, MAO Duo-bin, YANG Gong-ming* (College of Food and Biological Engineering, Zhengzhou College of Light Industry, Zhengzhou 450002, Henan, China) Abstract: Sixty strains of doubtful lactic acid bacteria isolated from the meat in low temperature in 3 days by dilution plate. After two times of lines separation, and then Gram staining, microscopic examination, catalase test, gelatin liquefaction experiments, nitrate reduction experiments and H S tests, at last thirty-six strains of 2 lactic acid bacteria were obtained. According to the morphological characters, physiological and biochemical properties, as well as chemical characteristics, thes