桑叶叶绿素光谱特征及其含量测定的初步研究.pdfVIP

/product/788.html 邓祥元, 高坤, 张丽娟 ( , 212018) [目的]研究桑叶叶绿素的光谱特征及其含量的变化规律[ 方法] 以 鲜桑叶为材料, 采用有机溶剂萃取法和分光光度法对桑 叶叶绿素进行提取与含量测定, 通过比较不同节位桑叶及桑叶不同部位的叶绿素含量, 分析桑叶叶绿素的光谱特征及其含量的变化规 律[ 结果]桑叶叶绿素的光谱特征与其他高等植物叶绿素的光谱特征类似; 不同节位桑叶中叶绿素含量为中部 底部 顶端, 但叶绿 素 a / b的比值却为底部 顶端 中部; 而桑叶不同部位叶绿素含量及 a /b 比值的高低顺序依次为叶柄 叶中 叶尖[结论] 不仅为叶 绿素的提取及含量测定提供了方法性参考, 而且有益于叶绿素的进 一步研究 叶绿素; 光谱特征; 分光光度法; 桑叶 S 888. 2 A 0517- 6611( 2010) 31- 17423- 02 Preliminary Study on SpectralCharacteristics and Content of Chlorophyll inMulberry Leaves DENG Xiang-yuan et a l ( Co llege of Bio echno logy and E nv ironm en a l E ngineering, J iangsu Un iversiy of Science and T echno logy, Zh en- jiang, J iangsu 212018) Ab s rac [O bjec ive] T he ami w as o fur h er explore spec ra l charac eris ics an d variance of chlorophy ll con en in m u lberry leaves. [M e h- od] Fresh m ulb erry leaves w ere u sed as raw m a erial in h is s udy, and chlorophyllw as ex rac ed by organ ic so lven ex rac ion process and m easuredw ih ul rav io le-v isible spec ropho om e ry. Ch lorophy ll con en s in differen node order and sec ion of m u lberry leaves w ere com pared and ana lyzed o ob ain sp ec ral charac eris ics and Var ian ce of ch lorophy ll con en . [ R esul ] I w as found ha sp ec ra l charac er is ics of chloro- phyll in m ulb erry leaves w ere smi ilar o h a in he o h er h igh er plan s leaves. The resul s also show ed ha he con en of chlorophy il in m iddle m ulberry leaf posi ion w as h e h igh es, h a in he bo om m u lberry lea f posi ion ranked h e second, and ha in h e op m ulberry leaf p osi ionw as he low es. H ow ever, h e order of chlorophy ll a / b va lue in differen node order of m ulb erry leavesw as bo om op m iddle. M oreover, fo r di-f feren m ulb ery leaf sec ioncs, he order o f chlorophy ll con en and ch lorophy ll a / b va lue w as p e iole leaf m iddle blade ip. [ C