甘肃乌拉尔甘草野生与栽培品内生菌的分离及鉴定_邓毅.pdfVIP

· 方 药 道地药材 Western Journal of Traditional Chinese Medicine,2012 Vol.25 No.11 甘肃乌拉尔甘草野生与 栽培品内生菌的分离及鉴定* 1 1 1 1 2△ 邓 毅 ，王 艳 ，丁仁伟 ，杨志军 ，张艳萍 1 甘肃中医学院，甘肃兰州730000 ；2 甘肃中医学院附属医院 ［摘 要］目的：通过对甘肃乌拉尔甘草野生与栽培品内生菌的分离，对甘草同属不同品种内生菌的优势菌 种进行初步鉴定，比较二者的生理活性差异。方法：采用NA 、PDA 培养基对甘草野生与栽培品内生菌进行分 离和纯化，并以分离频率比较判断各自优势菌种。结果：野生甘草分离到内生细菌65 株，内生真菌7 株，优势 菌种为青霉菌属和曲霉属；栽培甘草分离到内生细菌54 株，内生真菌6 株，优势菌种为青霉菌属；野生与栽培 甘草的内生真菌分属于6 个属，相同的有5 个属，不同的2 个属为丝核菌属和木霉属。结论：野生与栽培甘草 具有相趋同数量和种类的内生细菌与内生真菌，但二者内生真菌的菌属及优势菌种存在明显差异。 ［关键词］甘肃；乌拉尔甘草；野生栽培；内生细菌；内生真菌；分离纯化；种类鉴定 ［中图分类号］R282.7 ［文献标识码］A ［文章编号］1004-6852(2012)11-0008-04 Identification and Separation of Endophytes in Wild and Cultivated Glycyrrhiza Uralensis from Gansu Province 1 1 1 1 2△ DENG Yi , WANG Yan , DING Ren-wei , YANG Zhi-jun , ZHANG Yan-ping 1 Gansu University of Chinese Medicine, Lanzhou 730000, China; 2 University Hospital of Gansu Traditional Chinese Medicine AbstractObjective: To compare the differences of biological activity between wild and cultivated glycyrrhiza uralensis through the isolation of endophytes and identification on dominant bacteria from endophytes of different species in the same genera. Method: Endophytes from wild and cultivated GanCao were isolated and purified with NA and PDA base. The dominant bacteria were compared and judged with frequency separation. Result: All 65 strains of endophytic bacteria and seven strains of endophytic fungi were isolated from wild GanCao, the dominant bacteria were penic