玻璃苣醇提物对慢性抑郁模型小鼠+脑组织中神经递质及影响.pdfVIP

玻璃苣醇提物对慢性抑郁模型小鼠 脑组织中神经递质的影响 刚宏林，何志一，刘相辉 ( 哈尔滨市食品药品检验检测中心,黑龙江 哈尔滨，150525) [摘要]目的：通过建立慢性应激小鼠抑郁模型，观察玻璃苣对慢性应激抑郁模型小鼠脑内 5-羟色胺（5-HT）、去甲肾上腺素（NE）、多巴胺（DA）三种单胺类神经递质的影响，初步探 讨玻璃苣对抑郁模型小鼠的影响及其作用机制。方法：利用孤养和长期不可预见性温和应激 （CUMS）建立慢性应激小鼠抑郁模型采用酶联免疫吸附剂测定法（ELISA）测定慢性应激 抑郁模型小鼠脑内单胺类神经递质的变化。结果：模型组小鼠脑内5-HT、NE、DA含量明显 低于正常组小鼠；与模型组相比，玻璃苣醇提物高、中剂量组小鼠脑内5-HT、NE、DA的含 量均有升高，且差异有统计学意义。玻璃苣醇提物低剂量组小鼠脑内5-HT、NE、DA的含量 也有升高趋势，但差异没有统计学意义。结论：玻璃苣能够提高慢性应激抑郁模型小鼠脑内 单胺类神经递质（5-HT、NA、DA）的含量，玻璃苣对小鼠脑内神经递质的作用可能是玻璃苣 对抑郁模型小鼠产生影响的可能机制。 [关键词]玻璃苣、抑郁症、脑内单按递质 TheeffectsoftheethanolextractiveofBoragoofficinalisonneurotransmitter in the brain tissue of mouse model of chronic depression GANG Honglin, HE Zhi-yi Liu Xiang-hui （Harbin Food and Drug inspection center,. Heilongiang Harbin ,150525,china;2 Hematology department ,The first affiliated hospital of harbin medical university, Heilongiang Harbin 150001,china） 【Abstract】 Objective：After establishing chronic stress mouse models of depression，we studied on the effects of the extractive of Borago officinalis on norepinephrine（NE），dopamine（DA） and5-hydroxytryptamine（5-HT）inmousemodel of depression with chronic stress. Then discuss the prevention and cue effects of the extractive of Borago officinalis and the possible mechanisms. Methods： Established establishing chronic stress mouse models of depression，and using the enzyme-linkedimmunosorbentassay（ELISA）detectedthemonoamineneurotransmitters of the CUMS depression model. Results：The monoamine neurotransmitters（5-HT，NE， DA）contents in model mice were significantly less than normal group. The high， medium group of ethanol extractive from Borago officinalis all could heighten the contents of 5-HT、NA、DA of mice with chronic stress depression；and the low group of ethanol extractive from Borago officinalis approached a raising trend，but no statistical significance. Conclutions：The Borago off