应用分子印记固相萃取法结合高效液相色谱测定池塘水及三种鱼肉中磺胺甲基嘧啶含量.pdfVIP

Analysis of Sulfamerazine in Pond Water and Several Fishes by High-Performance Liquid Chromatography Using a Molecularly Imprinted Solid-Phase Extraction Guo Linyuan，Jiang Xiaoman ，Yang Cailing ，Zhang Haixia * Department of Chemistry ，Lanzhou University，Lanzhou （730000 ） E-mail ：zhanghx@ Abstract The synthesis and evaluation of a molecularly imprinted polymer (MIP) as a selective solid-phase extraction sorbent, coupled to high-performance liquid chromatography (HPLC), for the efficient determination of sulfamerazine (SMR) in pond water and three fishes are reported. The polymer was prepared using SMR as the template molecule, methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linking monomer in the presence of tetrahydrofuran as the solvent. The SMR-imprinted polymers and non-imprinted polymers were characterized by FT-IR and the static adsorption experiments. The prepared SMR-imprinted material showed high adsorption capacity, significant selectivity and good site accessibility. The maximum static adsorption capacity of the SMR-imprinted and non-imprinted material for SMR was 108.8 and 79.6 mg g-1, respectively. The relatively selective factor value of this SMR-imprinted material was 1.6. Several parameters influencing the solid-phase extraction process were optimized. At last, the SMR-imprinted polymers were used as the sorbent in solid-phase extraction to determine SMR in pond water and three fishes with satisfactory recovery. The limit of detection (LOD) for SMR was 1 ng g