抗菌丝素蛋白水凝胶敷料的制备.pdf

Material Sciences 材料科学, 2012, 2, 133-138 doi:10.4236/ms.2012.23024 Published Online July 2012 (/journal/ms) Preparation of Antimicrobial Silk Hydrogels for * Surgical Dressings 1 2 1 1 1# Xilong Wu , Yiyun Shen , Shan Sun , Xiu Zhang , Shenzhou Lu 1 National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 2College of Pharmaceutical Sciences, Soochow University, Suzhou Email: #lushenzhou@ Received: Jun. 25th, 2012; revised: Jul. 5th, 2012; accepted: Jul. 12th, 2012 Abstract: Using quaternary ammonium salts (QAS) as initiating agents, silk fibroin aqueous solutions were induced to gel rapidly. The structure, zeta-potential change and thermal stability properties of QAS-induced silk gel were charac- terized by means of X-ray diffraction (XRD), infrared spectroscopy (IR), scanning electron microscopy (SEM), zeta- potential measurements and differential thermal analysis (DTA). The results show that QAS-induced silk gelation is accompanied by the formation of β-sheets and less negative zeta-potential values. With the observation of SEM, the internal morphology of gel exhibited a porous three-dimensional network structure. In vitro degradation and DTA analysis revealed that QAS-induced fibroin gel was not quite stable and disintegrated more easily in the enzyme solu- tion. Also, QAS is amorphous or metastable crystal state in silk gel and thus easy to release and diffuse. The antibacte- rial test in vitro suggested that silk-QAS gel showed distinctly antibacterial activities against b