基于免疫分选的T淋巴细胞亚群嵌合性定量分析.pdfVIP

2011 1 21 1 Ch inese J urnal fH ea lth Lab rat ry Techn l gy, Jan 2011; V l 21 N 1 101 基于免疫分选的T淋巴细胞亚群嵌合性定量分析 许先国, 章伟, 陈舒, 朱发明, 吕杭军, 严力行 (, , 310006) [ ] : T : 14 + + , CD4 CD 8 T , 16 ( STR): T DNA STR , 10% , 16 STR, STR; 1% , , : T , [ ] 嵌合体; 免疫分选; 造血干细胞移植 [ ] R733. 7 [ ] A [] 1004- 8685( 20 11) 01- 0 10 1- 03 Quantitative chimerism analysis of T lymphocyte subsets based on immune sorting X U X ian - Guo, ZH AN G W ei, CH EN Su, ZH U Fa -M ing, L VH ang - j un, YAN L i- X ing ( B l d Center f Zhejiang Pr vince; Key Lab rat ry f B l d Safety Research, MOH, H angzh u 310006, China) [Abstract] Objective: T establish a quantitativem eth d f r ch mi erism analy sis f T lym ph cyte subsets based n mi m une s r ting. M ethods: 14 artificial ch mi era gr ups w ere m ixed acc rding t d ifferent lym ph cytes pr p rti n. The CD4+ and CD8+ T cell subsets w ere s rted by p sitive selecti n f mi m un m agnetic beads, and then were analyzed by STR p lym rph ism s tech n ique. Results: The DNA s extracted fr m s rted T cells were fit f r STR detecti n. A ll STR alleles specific f r recipient r d n r can be detected and the quantitative resu lts w ere c ns isten tw ith the retic values in ver 10% recip ient ch mi eras. But nly partial recip ient alleles can be detected and the quan titative results w ere d ifferent fr m the retic values in less then 1% recip ient chmi e ras. Conclusion: A quantitative ch mi erism analys is f T cell subsets based n mi m une s rting w as estab lished. Them eth d can be used t m nit ring f hem at p ietic ch mi erism f ll w ing hemat p ietic stem cell transplan tati n. [ eywords] Chmi era; Imm une s rting; H em at p