多烯磷脂酰胆碱对脓毒症大鼠的保护作用与PPARγ有关.pdfVIP

Intensive Care Unit, Sun Yat-sen University Cancer Center, Guangzhou 510060, China [Abstract] Objective To investigate the protective effects of PPC in rat CLP model and the underlying mechanisms. Methodes Prospective, randomized and controlled animal study was performed. Male Sprague-Dawley rats, weighed 200-250g were used in the study and all the surgeries were performed in SPF animal laboratory. The rats were randomly divided into five groups according to random number table : Sham group, Sham-PPC group, CLP group, CLP-PPC group and CLP-PPC/GW9662 group in which GW9662 was administrated before the pretreatment of PPC in CLP rats. The survival of CLP group and CLP-PPC group were monitored for 7 days after the introduction of sepsis. The rats were subjected to sepsis by cecal ligation and puncture (CLP). The hemodynamic parameters of all animals were recorded 18h after the surgery and the samples were collected. The arterial blood gas were analyzed and the level of AST, ALT, NO, superoxidase dismutase (SOD), Malondialdehyde (MDA) and TNF-α in serum were determined. HE staining was applied to observe the morphological changes of liver tissue while liver wet/dry ratio (W/D) were calculated simultaneously. Results Seven days after CLP, there were only 4 (4/20) rats left in CLP group while 12 (12/20) rats survived in CLP-PPC group. PPC could reverse the hypodynamic collapse in sepsis, with remarkably increasing mean arterial pressure (MAP, P=0.000), dP/dtmax (P=0.008) , dP/dtmin (P=0.003). Compared with CLP group, the rats in CLP-PPC group showed less severe hepatic inflammation. The level of liver injury parameters such as W/D (P=0.001), ALT (P=0.004), AST (P=0.000) were significantly lower in CLP-PPC group while blood glucose was sig