ABD活性因子提升抗癌药物效果的研究摘录.docVIP

7. Enhancing Effect of Patented Whey Protein Isolate (ABD活性因子?) On the Cytotoxicity of Anti-Cancer Drug Wayne Y. Tsai, Wen-Huei Chang, Ching-Hsein Chen, and Fung-Jou LuDepartment of Biochemistry, College of Medicine National Taiwan University, Taipei, Taiwan, R.O.C. ACCEPTED FOR PUBLICATION IN NUTRITION AND CANCER, VOL 38, ISSUE #2 Abstract:?To determine the enhancing effect of a whey protein isolate on the cytotoxicity of a potential anti-cancer drug. baicalein, human hepatoma cell line HepG2 was assigned to grow in different media for four days, followed by the investigation of cell growth and apoptosis. Excluding the control group with normal medium, other three treatment media included whey protein isolate (marketed as Immunocal) medium, baicalein medium, and combined medium containing both Irnmunocal and baicalein. MTT assay indicated that cells grew in combined medium had a significantly lower survival rate compared to the cells grew in baicalein medium; in contrast, for the cells grew in Immunocal group, there was no significant difference on survival rate. In the investigation of apoptosis. compared to the cells in baicalein medium, cells in combined medium showed a higher phosphatidylserine exposure, lower rnitochondrial transmembrane potential and nearly 13 times more cells were detected undergoing apoptosis. We also demonstrated that Immunocal was able to reduce glutathione in HepG2 by 20% to 40% and regulated the elevation of glutathione, which was in response to baicalein. In conclusion, Immunocal seemed to enhance the cytotoxicity of baicalein by inducing more apoptosis, this increase in apoptotic cells may be in association with the depletion of GSH in HepG2. This is the first study to demonstrate, in vitro, that Immunocal may function as an adjuvant in cancer treatments. [研究主题] 专利萃取美源康富ABD活性因子对抗癌药的细胞毒性有增强作用 [研究目的] 研究一种特殊的 [研究内容] 人体肝癌细胞HepG2分四组，在四种不同的培养基中培养四天后，测量其生长和凋亡的情况。对照组使用普通培养基，第二组使用ABD活性因子培养基，第三组使用黄芩甙元培养基，第四组使用ABD活性因子混合黄芩甙元培养基。 [研究结果] MTT分析结果显示