ATP生物荧光法在手卫生质量检测中的应用研究_刘波.pdfVIP

·1282 · Chinese Journal of Disinfection 2014 ;31 (12) 【论著】 ATP 生物荧光法在手卫生 质量检测中的应用研究 刘 波，张卫红，张苏明，张 翔，陈文森，李松琴 (南京医科大学第一附属医院，江苏南京210029) 摘要 目的 评价ATP 生物荧光法在检测手卫生质量中应用的可行性。方法 通过ATP 荧光检测仪及其配套试 剂，对某医院部分医务人员洗手效果进行检测与评价。结果 共检测32 例医务人员洗手前后的相对光单位值 (ＲLU)。医务人员洗手前ＲLU 数值四分位数P50 为484. 5 ，洗手后ＲLU 数值四分位数P50 为29. 5 ，平均手卫生质 量合格率为7 1. 88% ;男性医务人员手卫生质量合格率为61. 11% ，女性医务人员手卫生质量合格率为85. 7 1% 。 结论 ATP 生物荧光法用于检测医务人员手卫生质量具有可行性和实用性，操作简单，出结果快。 关键词 生物荧光法;手卫生质量;检测 中图分类号:Ｒ184 文献标识码:A 文章编号:1001 － 7658 (2014)12 － 1282 － 03 APPLICATION ＲESEAＲCH ON ATP BIOLUMINESCENCE ASSAY FOＲ HAND WASHING QUALITY DETECTING LI U Bo ，ZHANG Wei － hong ，ZHANG Su － ming ，ZHANG Xiang ，CHEN Wen － sen ，LI Song － qin (The First Affiliated Hospital with Nanjing Medical University ，Nanjing Jiangsu ，210029 ，China) Abstract Objective To assess the possibility of quality detecting of hand hygiene by ATP bioluminescence as- say. Methods ATP Hygiene Monitoring System with matching reagent was used to detect and analyze the hand washing effect of some medical staff in a hospital. Ｒesults Ｒelative light units (ＲLUs)of thirty － two medical staff before and after washing hands were detected. Quartile P50 before washing hands was 484. 5 ＲLU ，and quartile P50 after washing hands was 29. 5 ＲLU ，and average qualification rate of hand hygiene was 7 1. 88% ． Qualification rate of hand hygiene for male staff was 61. 11% and qualification rate of female staff was 85. 7 1% . Conclusion The quality detect of hand hygiene by ATP bioluminescence assay is possible ，practicable． This simple method is operated and results can be get fast. Key