CD4+CD25+调节性T细胞在胃癌患者的表达及其临床意义.docVIP

CD4+CD25+调节性T细胞在胃癌患者的表达及其临床意义 　　作者：雷晓,余佩武,赵永亮,石彦,饶芸　　作者单位：第三军医大学西南医院普通外科,重庆400038 　　提　要:目的　研究胃癌患者肿瘤组织、淋巴结及外周血中CD4+、CD25+调节性T细胞(T regulatory cells,Treg)的表达情况与胃癌临床病理的关系并分析其意义。方法　胃癌患者肿瘤组织、淋巴结、外周血及对照组中Treg表达情况分别以冰冻切片荧光抗体染色、流式细胞仪进行检测,结合胃癌患者临床病理情况分析。结果　健康成人对照外周血Treg表达率为(4. 1±5. 7)%,胃癌患者外周血Treg表达显著增高(16. 8±7. 9)%。胃癌组织Treg表达为(6. 5±7. 2)%,胃癌周围淋巴结Treg比例为(8. 4±7. 2)%,均较对照组显著增高。胃癌外周血和淋巴结Treg表达与淋巴结转移率和TNM分期有着负相关性,淋巴结转移阳性和TNM分期越晚,Treg表达率越高。结论　Treg在胃癌患者的瘤组织、淋巴结及外周血中表达较对照组显著增高,并与临床病理存在显著相关性。 　　关键词:胃癌,调节性T细胞 　　Abstract:Objective　To investigate the levels ofCD4+and CD25+regulatory T cells(Treg)and itsclinical significance in gastric cancer patients.Methods　Tumor tissue,para-gastric lymph nodes and peripheralbloodwere used to detect the levels ofTregwith immunofluorescence and flowcytometry. Another10 samples of lymph nodes from benign gastric diseases served as contro.l The Treg expression levelswere analyzedwith clinicalpathological information.Results　CD4+andCD25+cellswere observed in allgastric tissues andlymph nodes from gastric patients and controls. Te expression level ofTreg in control peripheral blood was(4. 1±5. 7)%,much lower than that[(16. 8±7. 9)%]of gastric cancer patients. Treg level in gastriccancer tissue and lymph nodes were(6. 5±7. 2)% and(8. 4±7. 2)%,both higher than that[(1. 8±2. 2)%, (1. 5±3. 1)%]in contro.l The expression levelofTreg in gastric cancerperipheralblood and lymphnodewere correlated negatively with lynph node metastasis and TNM classification.Conclusion　Treg levelrose significantly in gastric cancer tissues and correlatedwith clinical informations,indicating the immune stateofgastric cancer patients. 　　Key words:stomach carcinoma;T regulatory cell 　　调节性T细胞(T regulatory cells,Treg)是1995年Sakaguchi等[1]首先报道,在小鼠外周组织淋巴组织(脾脏、肠系膜淋巴结)等部位中存在具备特殊负调节功能的淋巴细胞,表达IL-2受体Α链(CD25),占CD4+T细胞比例约为5% ~10%,能以抗原非特异方式抑制效应T细胞活化,被称为天然发生的抑制性细胞(natural accruing T regulatory cells)。此后发现人外周血中存在免疫表型特点为CD4+CD25+CD45RO+的Treg[2]。研究表明,Treg有维持免疫稳态的重要作用,其数量和功能的异常可能对机体造