PCR检测宫颈病变组织和配偶尿道分泌物HPV的研究.docVIP

PCR检测宫颈病变组织和配偶尿道分泌物HPV的研究安徽医科大学学报 1999年第3期第34卷 基础医学研究作者：李京培　王明丽　史百芬　乔慕华　范晓芳　胡　勇　黄升海　陈禹保单位：李京培　王明丽　史百芬　胡　勇　黄升海　安徽医科大学微生物学教研室，合肥　230032；乔慕华　安徽医科大学附属第一医院妇产科，合肥　230022；范晓芳　合肥市第二人民医院妇产科，合肥　230011；陈禹保　北京美迪科生物工程公司，北京　100012关键词：宫颈疾病；聚合酶链反应；乳头状瘤病毒；人；肿瘤病毒感染　　摘要　目的　检测宫颈病变组织人乳头瘤病毒(HPV)感染对其配偶泌尿生殖道的受染情况。方法　应用聚合酶链反应对43例女性宫颈病变组织和配偶尿道分泌物进行HPV 6.11型和HPV 16.18型检测。结果　宫颈病变组织和配偶尿道分泌物HPV 6.11型检出率分别为27.91%和11.63%；HPV 16.18型检出率分别为51.16%和37.21%。配偶尿道分泌物与宫颈病变组织HPV 6.11同型检出率为41.67%，HPV 16.18型同型检出率72.73%。结论　女性感染HPV的同时，其配偶也有潜伏感染，表明HPV可通过尿道传播。 　　中国图书资料分类法分类号　R711.22Human papolloma virus infections in urinary genital　　tract of female patients and their partners by PCR techniqueLi Jingpei, Wang Mingli, Shi Baifen et al　　(Dept of Microbiology, Anhui Medical University, Hefei　230032)　　Abstract　Objective　To measure the positive rates of human papilloma virus (HPV) type 6.11 and 16.18 in discharges of 43 patients from female lower genital tract and in secretions of 43 their partners.Methods　HPV DNA in samples was quantitatively detected by polymerase chain reaction (PCR).Results　The positive rate in female pathological specimen and in secretions of their spouses were 27.91% and 11.63% for HPV 6.11 and 51.16% and 37.21% for HPV 16.18 respectively. The coincidence rates for HPV 6.11 and HPV 16.18 in female pathological tissues and in secretions were 41.67% and 72.73% respectively.Conclusion　When females suffer from HPV infection, their partners may have high probability to have an insidious infections, indicating the possibility of seminal transmission of HPV.　　MsSH　cervix diseases; polymerase chain reatoion; papilloma virus,human; tumor virus infections　　人乳头瘤病毒(human papilloma virus, HPV)除能引起传染性很强的生殖器感染外，还与生殖道上皮恶性肿瘤及癌前病变密切相关〔1，2〕，因此HPV的检测对尖锐湿疣、癌前病变及宫颈癌的早期诊断和防治有重要意义。为了解配偶双方HPV相互感染的关系，本研究采用聚合酶链反应(PCR)检测宫颈病变组织与其配偶的尿道分泌物中HPV基因型状况，为分子流行病研究提供依据。　　1　材料和方法　　1.1　标本　由安徽医科大学第一附属医院和市二院妇产科门诊提供，共有阴道镜检查异常病变组织43例，其中Ⅱ～Ⅲ度宫颈糜烂的慢性宫颈炎24例，尖锐湿疣16例，慢性宫颈炎3例；其对应配偶尿道分泌物43例。　　1.2　标本的采集和处理　用无菌棉拭子取宫颈脱落细胞或配偶尿道分泌物悬浮于无菌