ProPlus图像分析软件定量鸡胚尿囊膜血管新生面积的方法.docVIP

医学医疗：Image?Pro Plus图像分析软件定量鸡胚尿囊膜血管新生面积的方法 2009-5-12 15:35:58 点击： QQ次 发送给好友 和学友门交流一下 收藏到我的会员中心 保过辅导 免费试听 考试用书 考试宝典 经验交流模拟考试 - 【摘要】? 目的 应用Image图像处理和分析软件，研究鸡胚尿囊膜 (chick chorioallantoic membrane，CAM) 血管新生面积定量的新方法。方法 20只发育良好的7日龄鸡胚，分为龙葵给药组和对照组，每组10只。将中药龙葵水提液及等量蒸馏水吸附于5 mm直径的定性滤纸，置于CAM上。利用Image软件，定量血管新生面积、蛋壳开窗处对应的CAM面积，计算出血管新生面积与CAM面积的比值。结果 用Image可方便、自动、准确地进行给药前后的数据收集和面积计算。统计分析表明，受试物龙葵可明显抑制CAM血管新生，与对照组比较有极显著差异(P0.001)。结论 Image图像处理和分析软件是一种高效、准确的统计血管新生面积的工具，用该软件定量蛋壳开窗部位下的血管新生面积占开窗部位所对应的CAM总面积，不仅操作简便，而且数据计算自动生成，可较准确地反映鸡胚血管新生情况。 【关键词】? 图像分析 血管新生定量 鸡胚尿囊膜 ??? Quantitation of Angiogenesis in Chick Chorioallantoic Membrane ??? Using Imageoftware ??? XU Yang1，ZHAO Ying，BI Ming，LIU Yan3 ??? (1.Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences and Peking ??? Union Medical College，Beijing 100094，China; 2.Institute of information on Traditional Chinese Medicine， ??? China Academy of Traditional Chinese Medicine，Beijing 100700；3.Beijing University of Chinese Medicine，Beijing 100029) ??? 【Abstract】? Objective? To evaluate the value of method for quantitation of angiogenesis in chick chorioallantoic membrane (CAM) using Image，and the control group applied with the same volume of distilled water，respectively，absorbed on a piece of filter paper. Image，the total CAM area under the egg shell opening，the ratio between vascular and CAM area. Results? ImagePro Plus 5.0 software could be used to delineate and calculate the angiogenesis area and CAM area under the open egg shell window automatically and accurately. The angiogenesis was significantly reduced in the Solanum nigrumtreated CAM compared with that in control group (P0.001). Conclusion? ImagePro Plus 5.0 is an effective tool for evaluation of angiogenesis in CAM models．The percentage of angiogenesis area vs. CAM area can be used to reflect angiogenesis in CAM models simply and quatitatively. ??? 【Key words】? Image analysis，Software，Image，angiogenesis; Model，Chick chorioallantoic membrane ??? 鸡胚尿囊膜 (