TLR21在斑马鱼免疫功能中的作用研究.pdfVIP

, Jou rn al of A nhu i A gr.i Sci. 20 10, 38( 3 ): 1258 - 1260 姜丽 卢瑶 TLR21 欧阳蒲月, 郭成栓 ( , 510520 ) [目的] 研究斑马鱼 TLR21受体家族的作用[ 方法] 用各种纯组分刺激斑马鱼, 研究斑马鱼 TLR21受体家族 ( zTLR21. 1 zTLR21. 3zTLR21. 4 )的表达 变化, 推测它们的功能[结果] zTLR21. 1对于LTA刺激无反应, 对于LPSp loyI: C 和G lucan刺激都有上 调反应zTLR 21. 3 和 zTLR21. 4 对于 LPSLTA 和 p loyI: C 刺激均无反应, 对于G lucan刺激有明显上调变化[结论] zTLR 21. 1可能是一 种能够识别大部分抗原的模式识别受体zTLR21. 3和 zTLR21. 4的功能很类似, 可能都是酵母的特异识别受体 TLR21 TLRs 斑马鱼 Q 959. 4 Q 392. 11 A 0517 - 6611( 2010) 03- 01258- 03 Study on the Function of TLR21 in Immunity of Zebrafish OUYANG uyue et al ( Gu angdong Food and D ru g V ocat iona l Co llege, G uan gzh ou, G uangd ong 510520) Abstract [O bject ive] T he ami w as to study th e funct ion o f zeb raf ish TLR21 recep tors. [M ethod] Zeb ra fish w as stmi u lated w ith d ifferent pure com p on ents, then the variat ion of zeb raf ish TLR21 recep tors ( zTLR21. 1, zTLR 21. 3, zTLR21. 4) in expression w as stud ied to specu late the fun ction. [ R esu lt] Th e resu lts show ed th at LPS, p loyI: C and G lu can cou ld upregu late the exp res sion of zTLR21. 1 bu t not LTA. In addi t ion, G lucan cou ld obv iou sly u pregu late the expression o f zTLR2 1. 3 an d zTLR21. 4 bu t n ot LPS, LTA and p loyI: C. [ Con clu sion] zTLR21. 1 m igh t be a recep tor to recogn ize m ost of m icroorgan ism s, fun ction s of zTLR21. 3 and zTLR21. 4 are smi ilar, th ey m igh t b e spec if ic recep tors of yeast. Key words TLR21 TLR s Zeb raf ish T oll( T olllike receptors, T LR s) RNA ( dsRNA ) , , , TLR3, TLR3 , ( NF) B , [ 9] dsRNA T LR , , , [ 1] T oll , , , , T oll , ( )