乙型肝炎表面抗原124aa分子在大肠杆菌中的高效表达.pdfVIP

15 3 1999 7 I M M U N O LO G ICAL JO U RN A L Vo l 15 N o 3 J uly 1999 124aa 1 1 1 1 1 1 房德兴　李法卿　谭维国　陈 标　金慧英　李素芹 ( HBsA g ) 124 , 1 6 20 , 1617 8Da , , 22 % HiT r ap , 95% HBsA g HBsA g a E L ISA , HBsA g 392 1 R EXP RE SSIO N OF T HE HE PA T IT IS B SU RFA CE A NT IGE N F RA G M EN T W IT H 124 A M IN O A CIDS IN E coli Fang Dex ing, Li Faqing, Tan Weiguo, Chen Huabiao, Jin H uiying, Li Suqin ( , 210002) H uadong Research Institute of edical Biotechnics Nanj ing AbstractT he g ene frag m ent coding t he 124 am ino acids o f the car box yl ter m inu s o f hepatitis B sur f ace anti- gen ( HBsAg ) w as amplif ied by polym er ase ch ain reaction ( P CR) and the shor ted HBsAg w as ex pr essed in E coli in a fu sion pr otein f ashion T he fu sion pr otein w a s 16 17 8Da in molecular w eig ht and contained a 20-am ino -acid fu sion por tio n at it s am ino ter m inu s w hich co nt ained a six -histidine peptide seg m ent T he ex pressed pr ot ein s ag gr egated into inclu sio n bo dies w eig h abo ut 22 % o f the tota l b acteria pr otein s It w as easily purif ied w it h t he HiT rap aff init y m atr ix w ith a purity up to 95 % E L ISA detectio n sho w ed th at the pr o tein could be r ecog nized by po ly clo na l anti- bo dies against HBsA g and mo noclo nal antibo dy ag ain st HBsA g a deter m inant , , , Key words Hep atit is B v ir us Hep atitis B sur f ace a nt igen E coli Gene ex pression , , a HBsA g 124 , , HBsA g a ( HBsA g) ( M cA b) HBsA g , ,