冠心合剂对心梗后大鼠VEGF表达的影响_祝光礼.pdfVIP

第 29 卷 第 2期 Vol. 29 No. 2 2 0 1 1年 2 月 CH INESE ARCH IVES OF TRAD IT OI NAL CH INESE M ED CI INE Feb. 2 0 1 1 VEGF 祝光礼, 钱宝庆, 周凡, 刘昭 (, 310007) : 目的:观察冠心合剂对急性心肌梗死(AM I)大鼠 管内皮生长因子 (Vascular endot elial grow t fac- tor, VEGF)表达的影响方法: 结扎冠状动脉左前降支法建立大鼠急性心肌梗死模型, 观察冠心合剂对心梗后大 鼠 清VEGF浓度心肌 VEGF蛋白表达及梗死面积的影响结果: 冠心合剂治疗组及阳性对照组 清VEGF 浓 度及心肌 VEGF蛋白的表达增加, 高于模型组和假手术组(P 0. 05) ; 随冠心合剂剂量的增加, 梗死面积逐渐减 小; 冠心合剂大剂量组疗效与西药对照组无明显差异(P 0. 05) 结论: 冠心合剂可能通过促进心肌梗死大鼠心 肌 VEGF蛋白表达分泌, 从而促进梗死心肌 管新生 : 冠心合剂; 心肌梗死; 管内皮生长因子;促 管新生 : R285. 5 : A : 1673- 7717( 2011) 02- 0247- 03 E ffects of Guanxin D ecoction on the E xpression of VEGF in Rat of A cute M yocard ial In farction ZHU Guang-l,i Q IAN Bao-qing, ZHOU Fan, LIU Z ao ( Departm ent of Cardiology, Guangx ing H ospitalA ffiliated to Z ejiang C ineseM edicalUniversity, H angz ou 310007, Z ej iang, C ina) Abstrac:t O bj ectiv e: To investigate t e effect of Guanxin Decoction on m yocard ial angiogenes is and t e express ion of VEGF in ratsw it acutem yocardial infarction.M ethod s: Left anterior descend ing coronary arteries ( LAD) ave been liga- ted in SD rats after anest etized by 2. 5% pentobarb ital sod ium. T e level of VEGF in blood serum w asm easured 2w eeks after t e expermi ent by ELISA respectively. T e eart specmi enw ere fixed, sectioned and colorized by m eans ofH E stai- ning and mi m uno istoc em ical stain ing to observe VEGF t roug m icroscope. R esults: Guanxin Decoction - treatment group and positive control group w ere ig er t an t e m odel group and t e s am - operation group in t e expression of VEGF protein (P 0. 05). T e level of VEGF in b lood serum increased