几丁聚糖—胶原再生室和双桥接技术修复兔面神经缺损的实验研究.docVIP

几丁聚糖—胶原再生室和双桥接技术修复兔面神经缺损的实验研究 几丁聚糖mdash;胶原再生室和双桥接技术修复兔面神经缺损的实验研究 作者：屈振宇 杨聪 王卫红 许彪 作者单位：1.昆明医学院附属口腔医院 口腔颌面外科，云南 昆明 650031； 2.大连市口腔医院 口腔外科，辽宁 大连 116021 【摘要】 目的 探讨利用双桥接技术和几丁聚糖mdash;胶原再生室修复兔面神经长段缺损的可行性。方法 将实验用30只健康中国大耳白兔随机分成3组，分别为双桥接组（Ⅰ组）、原位神经吻合组（Ⅱ组）、正常空白对照组（Ⅲ组）。手术制作兔右侧面神经下颊支15 mm缺损模型，采用双桥接和原位神经吻合进行修复。术后12周进行大体观察、神经电生理检测、组织学观察、图像分析，以评价神经修复情况。结果 12周时，Ⅰ组几丁聚糖mdash;胶原再生室吸收明显，且无异物反应，能抑制周围纤维结缔组织形成，可为神经再生提供良好的微环境。Ⅱ组吻合口处膨大，周围有瘢痕样组织包绕。Ⅰ组神经肌肉功能恢复良好，神经传导速度（NCV）和复合肌肉动作电位振幅（M波）检测结果经统计学分析显示与Ⅱ组相似（P0.05），但仍未恢复至正常水平（P0.01）。结论 双桥接技术修复长段神经缺损方法简单、效果肯定。几丁聚糖mdash;胶原再生室生物相容性良好，适于体内植入修复神经缺损。 【关键词】 神经再生 双桥接技术 几丁聚糖mdash;胶原再生室 　　The experimental study of double-bridging facial nerve defect in rabbits by chitosan-collagen chambers QU Zhen-yu1，2, YANG Cong1, WANG Wei-hong1, XU Biao1. （1. Dept. of Oral and Maxillofacial Surgery, College of Stomatology, Kunming Medical University, Kunming 650031, China; 2. Dept. of Oral and Maxillofacial Surgery, Dalian Stomatological Hospital, Dalian 116021, China） 　　 [Abstract] Objective To evaluate the feasibility of repairing the long defects in rabbits by double-bridging technique and chitosan-collagen chambers. Methods Thirty Chinese rabbits were divided into three groups randomly. 15 mm defects in right facial nerve of rabbits were repaired in three groups: double-bridging（groupⅠ）, nerve auto-grafts（group Ⅱ）, and normal control（group Ⅲ）. General observation, electrophysiological study, histological study and image analysis were performed 12 weeks postoperatively. All results were used to evaluate the nerve regeneration.Results Chitosan-collagen chambers were obviously degraded 12 weeks postoperatively and there was no foreign body reaction at this stage. They also restrain the formation of neuroma and provided a good microcircumambience for nerve regeneration. The recovery of nerve regeneration were good in group Ⅰ. The nerve conduction velocity（NCV） and compound muscle action potential（CMAP） of regenerated nerve were an