苦草（Vallisneria+natans）对沉积物微生物群落结构的影响-.pdfVIP

J.Lake Sci.(湖泊科学),2015,27(3):445-450 http:// .E-mail:jlakes@ ©2015 by Journal of Lake Sciences 苦草(Vallisneria natans)对沉积物微生物群落结构的影响∗ 张亚朋 ,章婷曦1 1,2,3∗∗,王国祥1,2,3 (1:南京师范大学地理科学学院,南京210023) (2:江苏省碳氮循环过程与污染控制重点实验室,南京210023) (3:江苏省地理信息资源开发与利用协同创新中心,南京210023) 摘　 要:模拟湖泊系统构建了“沉积物 水 苦草(Vallisneria natans)”系统,应用磷脂脂肪酸(PLFAs)法测定在沉水植物苦 草不同生长时期沉积物表层微生物群落结构的变化,探讨沉水植物对沉积物中的微生物群落结构的影响.结果表明,从 苦草生长初期到旺盛期再到衰亡期,沉积物中有机质含量先下降后上升;总磷、有机磷、无机磷分别下降了8.97% 、 7.81% 、10.28% ;沉积物微生物的活性与总磷呈极显著负相关,在苦草生长初期和旺盛期,实验组的沉积物微生物活性大 于对照组,而在衰亡期对照组的沉积物微生物活性略高于实验组;不同时期沉积物中微生物群落结构发生了明显变化, 组成结构差异显著,微生物组成中细菌占主要成分(占微生物总量的76%~84% );细菌中革兰氏阳性菌占主要优势,且 革兰氏阳性菌百分含量随苦草生长呈上升趋势,革兰氏阴性菌呈下降趋势;真菌的百分含量呈上升趋势. 关键词:沉积物;苦草;微生物群落结构;磷脂脂肪酸 Influence of Vallisneria natans on microbialcommunity insediments 1 1,2,3 1,2,3 ZHANG Yapeng ,ZHANG Tingxi WANG Guoxiang (1:School of Geography Science,Nanjing Normal University,Nanjing210023,P.R.China) (2:JiangsuProvincialKey Laboratory of Carbon andNitrogen CycleProcess and Pollution Control,Nanjing210023,P.R. China) (3:Jiangsu Centerfor CollaborativeInnovation in GeographicalInformationResourceDevelopmentandApplication,Nanjing 210023,P.R.China) Abstract:A sediment-water-Vallisneria natans system was built within a simulating lake system,where phospholipid fatty acids (PLFAs) method wasused todeterminethechangesinsedimentmicrobialcommunity structuresinthesubmergedplant V. natans at different growth stages. The results showed that during the growth of V. natans,the organic matter content decreased first and then increased. The concentrations of total phosphorus, organic phosphorus and inorganic phosphorus decreased by 8.97% , 7.81% and 10.28% ,respectively,duringthegrowthperiod. Themicrobialactivityandtotalphosphorusinsedimentsshowedex- tremely significantly negative correlation. In the early exuberant period,microbial activity in the experimental group was greater than the control group,while the decline