自体骨髓间充质干细胞复合去抗原牛松质骨载体修复兔桡骨节段性骨缺损_江兵.pdfVIP

解剖与临床 　2005 年第 10 卷第 3 期 　Anatomy and Clinics Vol . 10 No . 3 195 自体骨髓间充质干细胞复合去抗原牛松质骨载体 修复兔桡骨节段性骨缺损 1 2 2 2 江兵 　孔荣 　朱六龙 　张文志 ( ) ( ) 【摘要】　目的:探讨以自体骨髓间充质干细胞 MSCs 复合去抗原牛松质骨载体 BCB 修复兔桡骨节段性骨缺损的 效果 。方法 :抽取 20 只新西兰大白兔的骨髓并分离 、培养 、扩增和建立诱导MSCs ,将其和BCB 复合 。手术建立兔双 桡骨干 15mm 骨骨膜缺损的动物模型 ,实验侧植入 MSCsBCB ,对照侧植入 BCB ,通过 X 线 、组织学检查等手段观察 不同时相的骨缺损修复情况 。结果 :在第 12 周时 ,实验侧骨缺损得到骨性修复 ,对照侧则未能修复 。结论 :MSCs 在 体内能成骨 ,MSCsBCB 能有效地修复节段性骨缺损 。 【关键词】　间充质干细胞 ;去抗原牛松质骨载体 ;骨缺损 ;修复 ( ) 【中图分类号】　R318 　　【文献标识码】　A 　　【文章编号】　1671 - 7163 2005 03 - 0195 - 04 Repairing Radial Segmental Defects in Rabbits with Mesenchymal Stem Cells and Antigen - extracited Bovine Cancellous Bonecarrier J iang B ing , Kong Rong , Zhu L iulong , et al . (Department of Orthopaedics ,Anhui Provincial Hospital ,Hefei 230001) ( ) 【Abstracts】　Objective :To study the efficacy of antigen - extracited bovine cancellous bonecarrier BCB loaded with autologous ( ) bone marrow mesenchymal stem cells MSCs on the healing of rabbit radial segmental defects. Methods :Autologous MSCs were isolated in vitro ,then implanted into the BCB . An experimental model of 15mm bone defect was made in the bilateral radius of 20 rabbits. In the experimental group ,the MSCsBCB was implanted into one radial segmental defect and the BCB was implanted into the other one . After the operation ,serial radiographic examination a