水稻基因组测序和分析的研究进展.pdfVIP

( ) , ( ) : 中国水稻科学 Chinese J Rice Sci 2004 , 18 6 557～562 557 水稻基因组测序和分析的研究进展 郭龙彪1 ,2 　程式华1 , 　钱 　前1 , (1 2 中国水稻研究所 水稻生物学国家重点实验室 , 浙江 杭州 310006 ; 浙江大学 生命科学学院 , 浙江 杭州 310029 ; 通讯联系人 , Email :shcheng @mail . hz . zj . cn ; qianqian188 @hotmail . com) Highlight s in Sequencing and Analysis of Rice Genome 1 ,2 1 , 1 , UO HENG IAN G Longbiao , C Shihua , Q Qian ( 1State Key L aboratory of Rice B iology , China N ational Rice Research Institute , Hangzhou 310006 , China ; 2 College of L fi e Science , Zhej iang University , Hangzhou 310029 , China ; Corresponding author , Email :shcheng @mail . hz . zj . cn ; qianqian188 @hotmail . com) Abstract : A highlight of rice genome sequencing and its information were presented . Till 2002 two working drafts for rice subspecies in dica and j aponica genomes and a highquality draft sequence for the j aponica had been produced . The initial assembly of the nonredundant se quences reached 389 . 81 - 409 . 76 Mb and there are around 32 000 - 56 000 genes. Less than 30 % of them have been annotated . There is a significant gradient in GC contents within a gene . Exon sizes are narrowly constrained , but intron sizes can be highly variable within and be tween organisms. The genome sequence differences between indica and j aponica rice are over 22 %. Nearly half of the predicted rice genes have no obvious homologs with A rabidopsis although there is substantial homology with available expressed sequence tags ( ESTs) in other cere als. Genome sequencing not only opened a new era for the life research -