穿心莲内酯对不同分化胃癌细胞株的体外增殖的影响_董建华.pdfVIP

2009 3 33 2 1 12 2 2 1 1 310053 2 : [ 目的] 明确穿心莲内酯对不同分化胃癌细胞株的体外增殖的影响[ 方法] 以不同浓度的穿心莲内酯处 低分化 ( M KN45) 中分化( SGC7901) 和高分化( M K N 28) 胃腺癌细胞株, 分别温育24h 48h 72h; 采用噻唑蓝比色法检测各和穿心莲内 酯对癌细胞的药物半数抑制浓度( IC50 ) , 比较穿心莲内酯对不同分化胃癌细胞株的体外增殖抑制作用的异同[ 结果] 穿心莲内 酯对胃癌细胞株M K N45SGC790 1M KN 28 的体外增殖均具有显著的抑制作用, 并且呈剂量效应和时间效应关系低分化的 M K N45 细胞株对穿心莲内酯的敏感性明显高于高分化的M K N 28 细胞株和中分化的SG C790 1 细胞株[ 结论] 穿心莲内酯对不 同分化程度的胃腺癌细胞株体外增殖抑制作用不同 : 穿心莲内酯; 分化程度; 胃癌细胞株; 敏感性; MT T 法 : R2855 : A : 2009) Effect of Andrographolide on Gastric Cancer Cell Lines in Various Degrees of Differentiation Dong j anhua, Y e za yuan, L shuguang, et al . Zhej iang Chinese M edic ine Univers ity , H angz hou ( 310053 ) China Abstract:[ Object ve] T o det er m ne the sens t v ty of A ndro gr aphol de n g astr c cancer cell l nes n var ous degr ees of d fferent a t o n. [ M ethods] T hree g astr c cancer cell l nes nclud ng M K N45( 1ow deg ree d fferent at o n) , SGC7901( moderate deg ree d fferent at o n) and M KN 28 ( h g h degr ee d fferent at o n) w ere ncubated w th A ndr og rapho l de of var ous concentr at on at 24 hour s, 48 hours, 72 hour s. T he opt al dens ty ( OD) value of each g ro up w as ex am ned by MT T test; compute the dat a to arr ve at nh b t on r at o( IR) and 50% nh b t ng concentr at on ( IC50 ) , make the sens t v ty d spar ty clear by co mpar e t he dat a. [ R esult s] A ndr og ra pho l de had s gn f cant nh b t on effects o n the pr ol ferat on of M K N45, SGC7901 and M K N 28 cells n v tr o, n a t me dependent and dose dependent way . Po or ly d ffer ent ated gastr c cancer cell l ne M KN45 s mor e sens t v ty to chemot her apy than w ell d ffer ent ated M K N