稀杯盔形珊瑚铜锌超氧化物歧化酶基因全长+cDNA序列的克隆与分析.pdfVIP

热带海洋学报 JOURNAL OF TROPICAL OCEANOGRAPHY 2015 年 第 34 卷 第 1 期: 83−89 海洋生物学 doi:10.3969/j.issn.1009-5470.2015.01.012 cDNA * 范程辉, 刘丽, 沈城, 郭昱嵩 524088 : PCR RACE Galaxea astreata (copper-zinc superoxide dismutase, CuZnSOD) cDNA , 730 bp5′(5′-UTR) 111 bp, 3′-UTR 188 bp, (open reading frame, ORF)471bp, 156 CuZnSOD , Cu2+His49His51His66 His123 , Zn2+His66His74His83 Asp86 , Cys60 Cys149 SignalP 3.0 Server , TMHMM Server v. 2.0 , PSORT II Prediction 56.5%, CuZnSOD : ; ; ; Galaxea astreata 中图分类号: Q785 文献标识码: A 文章编号: 1009-5470(2015)01-0083-07 Molecular cloning and sequence analysis of the copper-zinc superoxide dismutase gene in Galaxea astreata FAN Cheng-hui, LIU Li, SHEN Cheng, GUO Yu-song Fisheries College, Guangdong Ocean University, Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Zhanjiang 524088, China Abstract: The full-length cDNA sequence of copper-zinc superoxide dismutase (CuZnSOD) gene was obtained from Galaxea astreata using RT-PCR and RACE methods. The results revealed that the full-length cDNA of CuZnSOD gene was 730 bp including 111 bp 5′-UTR, 188 bp 3′-UTR and 471 bp open reading frame (ORF) encoding 156 amino acids. The deduced amino acid sequence contained two CuZnSOD protein family signals. Cu2+ integrated with His49, His51, His66, and His123, and Zn2+ integrated with His66, His74, His83, and Asp86. The only intrachain disulfide bond was formed between Cys60 and Cys149. The signal peptides were not predicted by SignalP 3.0 Server, the transmembrane domains were not predicted by TMHMM Server v. 2.0, and the possibility of 56.5% was located