肠胃清对多药耐药人口腔表皮癌细胞KBA1移植瘤和正常组织中阿霉素浓度的影响.docVIP

肠胃清对多药耐药人口腔表皮癌细胞KB-A-1移植瘤和正常组织中阿霉素浓度的影响 作者：孔令伟范忠泽 许建华 孙珏 朱美华 李琦 韩建宏 石晓兰 　　【摘要】 目的 观察中药复方肠胃清口服液对肿瘤多药耐药的逆转作用，以及对瘤组织和正常组织中化疗药物分布的影响，探讨中药增效减毒的作用机制。 方法 将具有多药耐药表型的人口腔表皮癌细胞株KB-A-1皮下注射接种于裸小鼠右侧腋下，随机分为5组:空白对照组，肠胃清组，阿霉素组，阿霉素+低浓度肠胃清实验药物组，阿霉素+肠胃清高浓度实验药物组。肿瘤称重、HPLC分析、流式细胞仪检测各组药物的抑瘤率、阿霉素在肿瘤组织与肝肾中的分布及肿瘤细胞的凋亡率。 结果 阿霉素和肠胃清单独给药均不能抑制肿瘤组织的生长，阿霉素与肠胃清联合治疗能显著减少肿瘤组织的重量，增加肿瘤组织内的阿霉素浓度，诱导肿瘤细胞发生凋亡。阿霉素与肠胃清联合治疗没有影响肝脏和肾脏组织阿霉素浓度。 结论 肠胃清在不影响正常组织阿霉素浓度的同时，通过增加阿霉素在肿瘤细胞内的积累而增强阿霉素的细胞毒性，从而抑制肿瘤组织的生长，逆转肿瘤的耐药。 　　【关键词】 肠胃清口服液;人口腔表皮癌细胞KB-A-1;多药耐药 　　Effect of Changweiqing oral liquid on the concentration of doxorubicin in KB-A-1transplanted tumor and normal tissue 　　KONG Ling-wei，FAN Zhong-ze，XU Jian-hua，et al. 　　Affiliated Putou Hospital，Shanghai University of TCM，Shanghai200062，China 　　【Abstract】 Objective To observe the effect of Changweiqing oral liquid（CWQ）on reversal of mulitdrug resistance and the effect on concentration of DOX（doxorubicin）in tumor and normal tissue.Methods The human oral epider-moid carcinoma cell lines KB-A-1，which were mulitdrug resistance，were injected o the right flank area of the nude mice，which were randomized into5groups:control group，CWQ group，DOX group，DOX plus CWQ（12.5g/kg）group and DOX plus CWQ（50g/kg）group.The tumor masses were weighted and the tumor inhibition rates were calculated;the concentration of DOX in tumor and normal tissue（livers and kidneys）were analyzed by high performance liquid chromatography;the apoptotic rate of cancer cells were detected by flow cytometry.Results The growth of tumors was not inhibited in DOX and CWQ groups，the weight of tumor masses were all smaller in DOX plus CWQ（12.5g/kg）group and DOX plus CWQ（50g/kg）group than that in other groups.The concentration of DOX in tumor and the apoptotic rate in cancer cell were all higher in DOX plus CWQ group than that in other groups.There were no significant difference between the concentration of DOX in livers and kidneys of every