胸腺内注射Fas抗体对心脏移植大鼠FasFasL表达的影响.docVIP

胸腺内注射Fas抗体对心脏移植大鼠Fas-FasL表达的影响 作者：王秋艳，满迎春，张国春，张晶敏 作者单位：秦皇岛港口医院心内科，河北 秦皇岛 066002；黑龙江省第二肿瘤医院；哈尔滨市第二人民医院；黑龙江省海伦市人民医院 　　【摘要】 目的：研究受体胸腺内注射Fas抗体对心脏移植大鼠Fas/FasL表达的影响。方法：应用大鼠同种异位腹腔心脏移植模型,将模型动物随机分为三组，每组8只动物。A组：移植前后不给予处置；B组：给环孢素A，按每日8mg/kg于术前1d口服；C组：胸腺注射Fas抗体，以0.1mg/kg剂量在术前注射。以免疫组化法和逆转聚合酶链式反应（RT-PCR）法观察术后移植心脏局部的Fas及FasL的表达情况。结果：与A组相比,受体胸腺内注射Fas抗体可减少移植心脏局部的Fas/FasL基因表达（RT-PCR法半定量结果P0.05）和蛋白表达（免疫组化法P0.05）。结论：受体胸腺内注射Fas抗体是一种有效的抑制心脏移植急性排斥反应的方法，可使移植心肌局部Fas/FasL表达下调。 　　【关键词】 抗原，CD95；心脏移植；移植物排斥 　　Influence of intrathymic injection of antiFas monoclonal antibody on FasFasL after heart transplantation/WANG Qiuyan,MAN Yingchun,ZHANG Guochun,ZHANG JingminDepartment of Cardiology，Qinhuangdao Port Hospital,Qinhuangdao,Hebei,066002,ChinaAbstract：Objective:To investigate the influence of intrathymic injection of antiFas monoclonal antibody on Fas/FasL of rats after heart transplantation.Methods:Using rat allograft heterotopic heart transplantation models，rats were randomly divided into three groups:group A(control group),group B(was treated with cyclosporin before test),and group C(was treated with intrathymic injection of antiFas monoclonal antibody before test). The number of rats of every group was eight.After operations，the samples were observed the express of Fas/FasL on the transplanted heart by RT-PCR and immunohistochemical method.Results:Compared to group A，intrathymic injection of anti-Fas monoclonal antibody significantly decreased the gene express (RT-PCR，semiquantitative) and protein level (immunohistochemistry) of Fas/FasL on the transplanted heart (P0.05).Conclusion:Intrathymic injection of anti-Fas monoclonal antibody has a immunosuppressive effect on acute reject reaction and down regulation on the express level of Fas/FasL after rat heart transplantation. 　　Key words：Antigens,CD95;Heart transplantation;Graft rejection 　　心脏移植后会发生免疫排斥反应,尤其是急性排斥反应[1]。控制心脏移植的急性排斥反应主要是应用免疫抑制剂。目前，国内、外常用的免疫抑制剂种类繁多且副作用较大，因此，寻找一种经济适用且副作用小的免疫抑制剂是医学界关注的话