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裙带菜多糖诱导人肝癌HepG_2细胞凋亡的研究.pdf
470 Acta Nutrimenta Sinica,Oct.,2007, Vol.29 No.5
ZOU Xiang-yangWANG Xue 1WANG Hua-xinGUO Lian-yingXING Rong HOU Lin 1
School of Basic Medicine, Dalian Medical University , Dalian 116027
1 College of Life Science ,Liao Ning Normal University,Dalian 116029,China
Abstract Objective: To investigate the inhibitory effect of Undaria pinnatifida polysaccharides
(UPPS) on hepatocellular carcinoma HepG-2 cells and its possible mechanism. Method: The effect of
inhibiting proliferation and inducting apoptosis of UPPS were determined by means of MTT and FCM. The
expression of Bcl-2 and Bax proteins was immunohis to chemcally evaluated after treatment of UPPS.
Results: UPPS inhibited HepG-2 cells growth in vitro , significantly higher than the negative control group
(P 0.01), and induced apoptosis after UPPS treatment 2448h, with decreased expression of Bcl-2 and
increased expression of Bax. Conclusion: UPPS inhibits the proliferation of HepG-2 cells by apoptosis,
perhaps associated with the change of expression of Bax and Bcl-2. [ ACTA NUTRIMENTA SINICA,200729
5470 472]
Key words Undaria pinnatifida polysaccharides (UPPS); hepatocellular carcinoma HepG-2 cells; apoptosis
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