雌兔阴蒂海绵体平滑肌细胞的体外培养及生物学特性.pdfVIP

1·98 · 第 10 卷 　第 3 期 中　华　男　科　学 Vol. 10 　No. 3 2004 年 3 月 National Journal of Androlo gy Mar. 2004 ·论 著· 雌兔阴蒂海绵体平滑肌细胞的体外培养及生物学特性 刘　波 , 刘继红 , 王　涛 (华中科技大学同济医学院附属同济医院泌尿外科 , 湖北 武汉 430030) 摘要 : 　目的 : 探讨雌兔阴蒂海绵体平滑肌细胞的体外培养方法及其生物学特性。　方法 : 采用酶消化法对阴蒂 海绵体平滑肌细胞进行体外培养 ,倒置显微镜下观察细胞形态 ,计数细胞贴壁率及生长情况 ,用免疫组织化学方法 鉴别培养的平滑肌细胞。　结果 : 体外培养的细胞证实为兔阴蒂海绵体平滑肌细胞 ,梭形 ,呈长轴平行排列 ,贴壁 快 ,生长迅速而平稳。　结论 : 体外培养的阴蒂海绵体平滑肌细胞可在合适的条件下生长和传代 ,并能够保持稳定 的生物学特性 ,可为阴蒂生理等方面的研究提供细胞材料。 关键词 : 阴蒂海绵体; 平滑肌细胞 ; 体外培养 ; 兔 ; 雌性 中图分类号: Q813. 1 ; Q954. 66 + 1 　　文献标识码 : A 　　文章编号 : (2004) Biological Characteristics of Female Rabbit Clitoral Smooth Muscle Cell Culture in vitro Liu Bo , Liu Jihong , Wang Tao Depart ment of U rology , Tongji Hospital , Tongji Medical College , Huaz hon g U niversity of Science and Technology , W uhan , Hubei 430030 , China ( L iu B , L iu J H , W ang T) Correspondence to : L iu Jihon g , Em ail :jhliu @tjh . tj m u . edu . cn ( ) Abstract : 　Objective : To investigate the method of culturing rabbit clitoral smooth muscle cells SMC in vit ro and their biological characteristics. 　Methods : Rabbit clitoral SMCs were cultured in vit ro by enzymatically dispersed method , and morphological obser vation , cell counting and immunohistochemical staining were employed to study their biological characteristics. 　Results : Rabbit cli toral SMCs were spindleshaped and parallelled along their longitudinal axis , and the attachment and proliferation in vit ro were rapid. 　Conclusion : Rabbit clitoral SMCs cultured in vit ro can g