溶酶体酶抑制剂对神经细胞内Nicastrin表达水平的影响.pdfVIP

彭雪堡笠鎏酶丝酶地制趔盟控经细胞凼盟i!塑尘!麦蓬盔垩数墅蛔．筮!!塑 ·2975· 溶酶体酶抑制剂对神经细胞内Nicastrin表达水平的影响 彭雪华1 龙志敏1 骆世芳1 贺桂琼1 (重庆医科大学儿童医院放射科，重庆400016) [摘要] 迹、亚细胞器的分级分离、免疫荧光双标等技术，检测溶酶体酶抑制剂处理后神经细胞内NcT的表达变化。结果亚细胞器分级分离实验显示，正 溶酶体内。结论神经细胞内mNcT的降篇与溶酶体途径有关。 (关键词]溶酶体酶抑制剂；Nicastrin；神经细胞；蛋白降解 【中图分类号]R749．1(文献标识码)A[文章编号] The ofNic鼬trinin肿uronalceⅡsafter inlIibitorIstreatment e印re鼹ionch锄ges lysosomal PENG Xue-Hua，LONGZlli-Min，LUo SlIi·F粕g，以口正 Medical 4(Hml6，Cllina X·RayDeparnllent，ChiIdren’sHospital，ChongqingUniVersity，Chongqing To whetherthe wasinvolvedinthe ofAlzheimer’s 【Abstract】objectiveexplore lysosomalp＆thway de铲adation lated Methods ofNCTstablecell methodssuchasWestem pIDteinNicastrin(NCT)．Followinggeneration lines，various andcell with inhibitionwereusedtocheckNCT levelinNCTstable immunonuorescentfractionation，combined staining lysosomal expression line． fhctionation showedthatNCTdistributed inER NCTlocatedin cell ResllltsCeU experiment p而marily and(知lgiapparatus，few lyso— increasedboth and mature NCTsta— some．‘I’reatment0fcellswith inhibitors NCT(mNCT)in lysosomal significantly endogenousexogenous cells neumnal theefEbctof inhibitoronmNCTwastime—and ble ornon—tmnsfectedcells，and 1ysosomal immature Immunonuorescem showedthat inhibitionleadedtothe inhibitorhadnoeffbcton NCT(imNCT)． microscopicandL丫sis lysosomal accumulationofNCTin CondusionsThe isinv01vedinthe ofmNCTinneuronalceUs 1vsosomal印p锄tus． 1vsosomalpathway degradation