动力学分光光度法测定中药多糖对羟自由基的清除率.pdfVIP

动力学分光光度法测定中药多糖对羟自由基的清除率 冯慧萍，杜瑞凝，张珍英(广东药学院分析化学教硼室．广州51000) 摘要：目的根据羟自由基与水杨酸【SA)氧化反应的动力学过程．建立动力学分光光度法测定中药多糖对羟自由基(·OH) 的清除率．方法应用紫外分光光度法，建立·OH与SA反应的动力学曲线，根据动力学曲线斜率的变化测定中药陈皮和灵 芝多糖对·OH的清除率，并用维生素C进行方法的重复性与可靠性验证．结果测定陈皮多糖对·OH的50％清除率(1c50) 为232．6 mg·L．1；灵芝IC50为O．8561 可靠，可用于中药多糖成分对·OH清除率的测定． 关键词：动力学分光光度法；羟自由基：水杨酸；淆除率 Determinationofthe ratioof to freeradicalKinetics EliminatingPoIysaccharidehydroxyl by pectrophotometry FENG Pharmaceutical ofanalyticalchemistry，Guangdong Hui-ping，DURui—ning，ZHANGZhen-ying(Department 510006) University，Guang：hou ABSTRACT：OBJECTI％’EThereaction of tadical acidwas elimination dynamichydroxyIf．OH)andsalicylic explored,the ratiosof Chinese medicineto‘OHwasdeterminedkinetic extractiveof traditional polysaccharide by curve eliminationratioof The ofthereaction·OHandsalic、-licacidWasbuildedUVmetho正The dynamics by tangerinepee andGanodermalucidum to·OHwasdeteminedbasedonthe ofthe polysaccharide potysaccharide slopechange and ofthismethodwastestedvitaminC．RESUJS11他50％elimination reliability by ratio(IC50)of graph．Repetitiveness extractiveof and lucidumto‘OHwas232．6 O．8561 polysaccharidetangerinepee Ganoderma mg’L“and g-L．‘respectively．the IC50ofvitaminCWas ThemethodWassensitiveand was 0．2544mmol·L～，RSDwas0．2％(刀=5)．CONCLUSl0N reliable,it to useddeterminetheeliminationratioof extractiveofChinesetraditionalmedicineto·