《Application of nonsmall cell lung cancer pleural effusion cell blocks in molecular pathological detection》.pdfVIP

Chinese-German J Clin Oncol April 2014, Vol. 13, No. 4, P157–P161 DOI 10.1007/s10330-014-1293-4 Application of non-small cell lung cancer pleural effusion cell blocks in molecular pathological detection 1 2 3 1 1 1 1 Ying Zhang , Nan Jiang , Dongdong Qian , Xiangzhou Li , Yu Zhou , Jia Mei , Xiaohui Cao 1 Department of Pathology, 81 Hospital of PLA, Nanjing 210002, China 2 School of Pharmacy, Nanjing Medical University, Nanjing 210029, China 3 Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing Stomatological Hospital, Nanjing 210008, China Received: 12 February 2014 / Revised: 25 February 2014 / Accepted: 10 April 2014 © Huazhong University of Science and Technology 2014 Abstract  Objective: The tumor tissues used in molecular pathological detection were usually obtained by surgery, which would cause trauma and may not be suitable for the terminal cancer patients. This paper evaluated the value of the non-small cell lung cancer (NSCLC) pleural effusion cell blocks as tumor tissues replacement materials in the application of molecular pathological detection. Methods: Tumor cells were made into cell blocks through stratified centrifugal from 30 NSCLC pa- tients with the pleural effusion. The immunohistochemistry, fluorescence in situ hybridization (FISH) and gene sequencing methods were employed in our experiments. Results: The tumor cells of cell block section were rich and could keep part of histological structure. Immunohistochemistry staining could assist diagnosis and tumor parting. Epidermal growth factor receptor (EGFR) FISH-positive was found in 33.33% of the group, high polysomy in 6 ca